To explore the anti-mutant effect of Codonopsis pilosula,the root tip of Vicia faba induced by 30 mg/L cyclophosphamide was cultured with different concentrations(0.01,0.05,0.10,0.15,0.20 g/mL) of water extract of Codonopsis pilosula,and the micronucleus was recorded,the content of reducing sugar,root activity,SOD activity,and MDA content were determined.The results showed that:Compared with the positive control(recovery culture by distilled water after 30 mg/L cyclophosphamide under treatment),different concentrations of Codonopsis pilosula decreased the micronucleus rate of Vicia faba root tip,increased the reducing sugar content of root tip,enhanced the root activity,increased the activity of SOD and decreased the MDA content under 30 mg/L cyclophosphamide treatment, when the concentration of Codonopsis pilosula was 0.05 g/mL,the inhibition rate of micronucleus in Vicia faba root tip was 35.41%,and the content of reducing sugar was the highest(2.81%),root activity was the highest[120.73 μg/(g·h)],SOD activity was the highest(88.44 U/g),and MDA content was the lowest(13.75 nmol/g).Therefore,Codonopsis pilosula had a certain anti-mutant effect on the damage of Vicia faba root tip cells induced by 30 mg/L cyclophosphamide, and the effect was the best when the concentration was 0.05 g/mL.