The “3414” experimental design was used to carry out a pot experiment,in order to study the effects of three trace elements of B(boron),Zn(zinc),and Fe(iron) foliar application on chlorogenic acid,ferulic acid,coniferyl ferulate,ligustilide and senkyunolide A accumulating of Ligusticum chuanxiong Hort.(Hereinafter referred to as Chuanxiong),which could lay a foundation for the formation of trace elements balanced fertilization techniques for chuanxiong.Results as follows:B was not conducive to the accumulation of coniferyl ferulate,ligustilide and senkyunolide A.Low concentration of B(1 g/L) could promote the accumulation of chlorogenic acid and ferulic acid,and the chlorogenic acid and ferulic acid content was 6.56% and 2.22% respectively higher than that without B.Excessive use of B had inhibitory effects.Zn was not conducive to the accumulation of chlorogenic acid,coniferyl ferulate,ligustilide and senkyunolide A.High concentration of Zn(4 g/L) could increase the ferulic acid content with 33.70% higher than that without Zn.The contents of coniferyl ferulate,ligustilide and senkyunolide A at low concentration of Fe(1 g/L) were respectively 5.34%,9.79% and 12.23% higher than those without Fe.The contents of chlorogenic acid and ferulic acid at high concentration of Fe(4 g/L) were respectively 8.46% and 6.98% higher than those without Fe.B and Zn had a negative interaction on the accumulation of 5 active ingredients of chuanxiong.B and Fe had a negative interaction on the accumulation of ferulic acid and a positive interaction on the other 4 active ingredients.Zn and Fe had a positive interaction on the accumulation of chlorogenic acid,ligustilide and senkyunolide A,and a negative interaction on the accumulation of ferulic acid and coniferyl ferulate.Therefore,foliar application of B,Zn and Fe fertilizers might not be effective in increasing the active ingredients content of chuanxiong.It was necessary to control the dosage so as not to have adverse effects.It should be not applied or as little as possible during cultivation.