In order to understand the function of wheat ABA receptor gene TaPYL9(Pyrabactin resistance like 9)under abiotic stress,TaPYL9 was isolated by homologous cloning method,the complete sequence was analyzed by bioinformatics method,and the expression levels of TaPYL9 were detected by real-time PCR under ABA,NaCl and PEG conditions.The results showed that full-length of TaPYL9 was 1 173 bp,and contained a complete open reading frame of 618 bp,encoding an unstable hydrophilic protein which was consisted of 205 amino acid residues.TaPYL9 protein was predominated by α-helix,and contained a
PYL helix structure which was composed of 2 α-helixes and 7 β-folds;TaPYL9 protein shared the highest protein sequence identity with AsPYL9-like protein in Aegilops tauschii,and it belonged to the same subfamily with AtPYL9 protein in Arabidopsis;TaPYL9 protein had the same motif with AsPYL9-like protein,and the motif similarity of TaPYL9 protein was the highest to AtPYL9 protein of all PYL proteins in Arabidopsis.Real-time PCR showed that the expression levels of TaPYL9 gene generally increased under the ABA,NaCl and PEG conditions. Overall,TaPYL9 protein is a wheat ABA receptor,which is sensitive to ABA, and maybe regulate salt and drought stress in wheat.