In order to improve the germination rate and plantlet regeneration rate of wheat haploid embryos,the induction rate and plantlet regeneration rate of haploid embryo from hybrid spike of different wheat materials × maize under different growth environments (culture in vitro, pot culture and field culture)and different media[1/2MS medium,named MC1; adding 50 mg/L vitamin C and 150 mg/L glutamine in 1/2MS medium,named MC2;adding 0.5 mg/L IAA (indole-3-acetic acid),0.5 mg/L KT(kinetin),150 mg/L glutamine in 1/2MS medium,named MC3]were analyzed.The results showed that environmental condition and culture medium had significant effects on the induction rate and plantlet regeneration rate of haploid embryo. The quality of haploid embryo obtained from wheat hybrid spike cultured in vitro was higher,the germination rate and plantlet regeneration rate of haploid embryo were 81.5% and 63.0%,respectively,which were 60.1% and 3.4 times higher than those from field culture,30.4% and 68.0% higher than those from pot culture.The culture effects of different wheat materials were the best on MC3,the average germination rate and plantlet regeneration rate of wheat haploid embryo were 42.3% and 15.3% on MC1,46.2% and 27.5% on MC2,and 65.6% and 38.2% on MC3,respectively.In summary,1/2 MS+0.5 mg/L IAA+0.5 mg/L KT+150 mg/L glutamine had good culture effect on the small and medium-sized embryos and immature embryos with poor development,and obtained relatively high germination rate and plantlet regeneration rate,but the rate of one-time seedling formation was low,so it was necessary to replace the medium for root-promoting seedling culture.