The homologous gene of Arabidopsis thaliana CER4 regulating wax synthesis and metabolism was cloned from South China cucumber 649 and named as CsCER4.The CsCER4 gene had a CDS region with 540 bp in length and encoded 179 amino acids.Promoter sequence analysis indicated that the CsCER4 promoter had regulatory elements related to various stresses such as drought,cold,and salt.Phylogenetic analysis showed that cucumber CsCER4 was closely related to CER4 of melon,bitter gourd,pumpkin and zucchini.The results of qRT-PCR showed that CsCER4 had high expression level in ovary and pericarp,and
its expression level was low in male flowers,stems and leaves.It was speculated that CsCER4 might directly participate in or affect the synthesis of wax by regulating the wax synthesis pathway.