Botrytis cinerea was a serious pathogen for plants. In order to provide the reference for studying pathogenic mechanism and control measures of Botrytis cinerea,the pathogenicity and host speciality of protein toxin secreted from Botrytics cinerea were determined with plants from 23 species in 16 families.The results showed that 17 species of plant were damaged by protein toxin,accounting for 73.91% of total plants,the pathogenicity of the toxin was stronger to Hippeastrum rutilum of Amaryllidaceae and Capsicum annuum of Solanaceae,and the lesion areas were respectively(2.18±0.44),(0.81±0.33) cm² after 7 days of inoculation,followed by Schefflera octophylla of Araliaceae,Dendranthema morifolium and Lactuca sativa of Compositae,Lycopersicon esculentum and Nicotiana tabacum of Solanaceae,Vicia faba and Pisum sativum of Leguminosae.The protein toxin could significantly inhibit seed germination and plantule growth of seven plants,such as Brassica pekinensis of Cruciferae,Vicia faba and Pisum sativum of Leguminosae,Lycopersicon esculentum of Solanaceae.Therefore,the host of Botrytis cinerea was wide,and its protein toxin was pathogenic with a non-host specificity.