To study the effects of hawthorn and Astragalus on the negative energy balance and immune function of perinatal dairy cows,blood biochemical and immune parameters of cows were measured after adding different doses of hawthorn and Astragalus.45 Holstein cows in 2—4 parity and healthy perinatal period(21 d before delivery—21 d after delivery) were chosed,and divided into group A,group B and group C randomly. Group A was fed a basic diet,groups B and C were added 100 g/(head·d) and 300 g/(head·d)hawthorn and Astragalus(hawthorn and Astragalus 1∶1),respectively.Blood was collected from the tail vein on 21 d (-21 d) before and 7 d (-7 d) before delivery,on the day of delivery(0 d),on 7 d(+7 d) and 21 d(+21 d) after delivery,and blood biochemical and immune parameters of cows in each group were measured.The results showed as followed:compared with group A,in terms of protein indicators,the total protein content of group B increased significantly by 44.10%,and globulin content increased significantly by 66.16% at +21 d.In terms of calcium and phosphorus indicators,the calcium content in group B increased significantly by 47.28% at +7 d.Calcium content increased significantly by 41.67% and phosphorus content increased significantly by 33.57% at +21 d.In terms of fat indicators,the content of non-esterified fatty acids in group C decreased by 27.16% at +7 d,and group B decreased significantly by 34.38% at +21 d.In terms of energy indicators,the glucose contents of group B and C increased significantly by 24.49% and 23.72% at 0 d,respectively.Ketone body content of group B decreased significantly by 25.93% at +21 d.In terms of immune indicators,the levels of immunoglobulin G in group B and C were significantly or extremely significantly increased(P<0.05 or P<0. 01),and the content of tumor necrosis factor alpha in group B was significantly or extremely significantly increased(P<0.05 or P<0.01) at -7 d,0 d,+7 d,and +21 d.In summary,the addition of 100 g/(head·d)hawthorn and Astragalus to perinatal dairy cow diets can regulate lipid, protein and calcium and phosphorus metabolism in postpartum dairy cows,reduce postpartum negative energy balance, reduce postnatal ketone body levels,and improve cow immunity.