The NBS-LRR family members of foxtail millet(Setaria italica)were identified,and their chromosome location,gene structure,conserved motif,phylogeny and expression level were analyzed,which laid a foundation for the application of NBS-LRR family genes in molecular breeding of disease resistance in Setaria italica.The results showed that 411 genes of NBS-LRR family were obtained,including 376 genes with CN(CC-NBS) structure,33 genes with CNL(CC-NBS-LRR) structure,1 gene with TIR(Toll interleukin-1 receptor) structure,and only 1 gene with NBS structure.Most of the NBS-LRR family genes were located on chromosome 8.There were four conserved sequences in the NBS domain of Setaria italica,and the family was divided into three groups in phylogeny.The gene structure was diverse,and Motif1 was the most conserved.Twenty-three NBS-LRR genes of Setaria italica were collinear with those of Setaria viridis,21 NBS-LRR genes of Setaria italica were collinear with those of Oryza sativa,1 NBS-LRR gene of Setaria italica was collinear with those of Arabidopsis thaliana.Among them, Seita. 8G088500 and Seita. 8G088400 were identified as high homologous genes to rice blast resistance genes.The Ka/Ks values of collinear genes between Setaria italica and Oryza sativa,Arabidopsis thaliana were less than 1,but among the collinear genes between Setaria italica and Setaria viridis,Ka/Ks values of Seita.6G014500 and Seita. 6G023500 were greater than 1.Expression profile analysis showed that the number of NBS-LRR genes highly expressed in the roots and panicles of foxtail millet was more,followed by the leaves,and the least in the shoots,indicating that the family genes may play an important role in the process of disease resistance in the roots and panicles.