Periconia igniaria Stdif10 is an endophytic fungus isolated from the root tubers of Stephania dielsiana Y.C.Wu for the first time. In order to understand the biological activity of P.igniaria Stdif10,the anti-insect,antifungal and antibacterial activities were determined with the extracts from petroleum ether layer,ethyl acetate layer and n-butanol layer of the fermentation products of this strain,by using the filter film method,mycelium growth rate method and toxic medium coating method.The results showed that petroleum ether extract possessed the best anti-insect and antimicrobial activity,having remarkable contact activity against Sitophilus zeamais with the LC₅₀ value at 2.295 1 mg/cm² after treatment for 72 hours,and having a good repellent activity to S.zeamais.After treatment for 48 hours,the repellent rate of 3.932,7.863,15.726 mg/cm² concentration gradients of petroleum ether extract to S.zeamais was greater than 62%. Moreover,the petroleum ether extract showed inhibitory activity against Colletotrichum capsica,Cochliobolus miyabeanus, Pestalotiopsis theae, Alternaria oleracea, Ceratocystis paradoxa, Phytophthora parasitica var.nicotianae,Exserohilum turcicum,Diaporthe citri,Alternaria citri to some extent,and had best antifungal activity against Colletotrichum capsica, Phytophthora parasitica var.nicotianae and Diaporthe citri with the EC₅₀ values at 0.117 3,0.071 6 and 0.054 7 mg/mL after treatment for 72 hours,respectively.The petroleum ether extract also exhibited significant inhibitory activity against Enterobacter aerogenes,Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Salmonella typhi, Staphyloccocus aureus,Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Bacillus anthraci with the MIC values ranging from 0.125 to 0.500 mg/mL after treatment for 24 hours.The results in this study showed that endophytic fungus Stdif10 had remarkable anti-insect and broad-spectrum antimicrobial activities,and the petroleum ether extract had the most significant activity(the polarity of the active substance was low).