Horticulture

Differential Expression Pattern of ANS Genes in Different Color Radish(Raphanus sativus L.)

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  • (Modern Agriculture and Bioengineering School,Yangtze Normal University,Fuling 408100,China)

Received date: 2019-08-10

  Online published: 2019-12-15

Abstract

To explore the relationship between expression level of ANS gene and pigment content in different color radish,sixteen radish varieties with different color fleshy roots were collected as materials and their expression levels of ANS gene were detected from cDNA samples using real-time PCR technology,as well as their pigments content were determined.Subsequently,the relationship between expression level of ANS gene and the content of pigment was explored.The results showed that,the content of pigment(F=114.294 0,P=0.000 1) and expression level of ANS gene(F=95.984 0,P=0.000 1)were both significantly changed in different color radish respectively. Especially it was found that the content of pigment and expression level of ANS gene were both significantly higher in red radish(23.80‰,6.439 0)than those in white radish(0.10‰,0.126 8); In addition,the correlation analysis between expression level of ANS gene and content of pigment in different color radish was conducted,and it was found that their correlation reached extremely significant level and correlation coefficient was 0.83.it was inferred that ANS gene might be one of key regulators in pigment synthesis of radish.

Cite this article

LIU Hongfang, CHEN Fabo, LI Wenbo, FANG Ping . Differential Expression Pattern of ANS Genes in Different Color Radish(Raphanus sativus L.)[J]. Journal of Henan Agricultural Sciences, 2019 , 48(12) : 98 -102 . DOI: 10.15933/j.cnki.1004-3268.2019.12.014

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