Agricultural Resources and Environment

Construction of a High Efficient Cellulose Degradation Composite Strains M6 and Its Compost Effect Analysis

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  • (1.Institute of Agricultural Product Quality Standard and Testing Research,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet 850032,China;2. College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,china)

Received date: 2019-05-27

  Online published: 2019-12-15

Abstract

In order to investigate the synergistic degradation effect of microbial complex strains on cellulose,six high-temperature cellulose degrading bacteria were screened from the farmland soil in Tibet and rice field leaf decay deposits in Changsha,and a high efficient cellulose degradation composite strains M6 was constructed.The effects of composite strains M6 on the temperature,pH value,total organic carbon content,total nitrogen content,carbon nitrogen ratio,total nutrient content and seed germination index of compost materials were analyzed.The results showed that,composite strains M6 treatmeat had better CMCNa enzyme activity and filter paper enzyme activity,When fermented 25 d at 50℃ ,the degradation rate of rape straw inoculated with composite strains M6 could reach 16.4%.The composite strains M6 treatment had higher compost temperature and longer high-temperature time than other treatments,the peak temper ature reached 63.7℃ on the 3rd day of fermentation,and the high-temperature period lasted for 8 days;The pH value of compost materials inoculated with composite strains M6 treatment showed weak alkalinity;at the end of composting,the content of total organic carbon in composite strains M6 treatment decreased from 50. 44% to 32.77%,and the content of total nitrogen increased from 1.79% to 2.28%,theC/N decreased from 28.18 to 14.19,and the total nutrient content was 13.27%.The GI value of the composite strains M6 treatment was always higher than other treatments,the GI value was 107% at the end of the compost,the composting treated with composite strains M6 had better permeability and quality.

Cite this article

LIU Qinghai, PAN Hu, ZHU Zhaojing, TIAN Yun, WANG Chong, DAWA Zhuoma, LU Xiangyang, BAI Junping . Construction of a High Efficient Cellulose Degradation Composite Strains M6 and Its Compost Effect Analysis[J]. Journal of Henan Agricultural Sciences, 2019 , 48(12) : 56 -62 . DOI: 10.15933/j.cnki.1004-3268.2019.12.009

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