Plant Protection

 Inhibitory Effects,Growth-promotion Evaluation and Identification of Two Biocontrol Bacteria against Fungal Diseases of Sesame

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  •  ( Institute of Plant Protection,Henan Academy of Agricultural Sciences/Key Laboratory of IPM of Pests on Crop (Southern North China) ,Ministry of Agriculture/Key Laboratory of Crop Pest Control of Henan Province,Zhengzhou 450002,China)

Received date: 2019-04-10

  Online published: 2019-11-15

Abstract

 In order to obtain effective and safe biocontrol resources to control sesame fungal diseases,SFB34 and SFB109 were screened from 412 soil samples.The antagonistic abilities to the pathogens(Mac rophomina phaseolina,Fusarium oxysporum,Bipolaris sorokiniana,Corynespora cassiicola and Nigrospora sphaerica) were detected through plate confrontation method and toxic medium method,and their promo tion evaluation and identification were carried out.The results indicated that the strains( SFB34 and SFB109) had good inhibitory effects on the five pathogens.The inhibition ratios of the fermentation fil trates of strains SFB34 and SFB109 ranged from 47.13% to 88.89% and from 38.84% to 86.90%,re spectively.The plant promoting ability was detected through root-drenching method. The bacteria resus pensions of 5×108 cfu/mL SFB34 and SFB109 could promote sesame seedlings growth significantly.Strain SFB34 increased the plant height,root length,fresh and dry weight of sesame seedlings by 8.91%,22.42%,28.02% and 23.52%,while strain SFB109 increased by 8.57%,13.16%,20.88% and 11.76%,respectivly.The screened biocontrol bacteria were identified by morphological observation,phys iological biochemistry and molecular criteria.It showed that SFB34 was classified into Bacillus velezensis and SFB109 was classified into Bacillus subtilis.This study provided a theoretical basis for further devel opment and utilization of SFB34 and SFB109 as biological control resources for sesame fungal diseases.

Cite this article

HE Bipo, ZHAO Hui, LIU Hongyan, NI Yunxia, WEN Yi, LIU Xintao .  Inhibitory Effects,Growth-promotion Evaluation and Identification of Two Biocontrol Bacteria against Fungal Diseases of Sesame[J]. Journal of Henan Agricultural Sciences, 2019 , 48(11) : 92 -98 . DOI: 10.15933/j.cnki.1004-3268.2019.11.013

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