&nbsp;Transcription Factor NtMYC2b Is Involved in the
 Formation of Heterosis of Nicotine Content Trait

WU Yuyao, XIE Ｒui, YANG Youcheng, LIU Kunhua, NIE Qiong

doi:10.15933/j.cnki.1004-3268.2019.11.007

Journal of Henan Agricultural Sciences >

2019 , Vol. 48 >Issue 11: 45 - 53

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.11.007

Crop Cultivation & Genetic Breeding

Transcription Factor NtMYC2b Is Involved in the Formation of Heterosis of Nicotine Content Trait

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( 1.College of Agriculture,Guizhou University，Guiyang 550025,China; 2.Guizhou Provincial Key Laboratory of Tobacco Variety Ｒesearch,Guizhou University,Guiyang 550025,China; 3.Zunyi Academy of Agricultural Science，Zunyi 563000,China; 4. Zunyi Tobacco Company,Zunyi 563000,China)

Received date: 2019-05-22

Online published: 2019-11-15

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Abstract

To investigate the correlation between transcription factor MYC2b and nicotine content and its heterosis in tobacco,ＲT-qPCＲ was used to analyze the expression of NtMYC2b and key enzyme genes in the nicotine synthesis pathway,and the nicotine content and its heterosis in roots and leaves of tobacco were determinated.The results showed that the expression of NtMYC2b in parent roots(GDH88 and Bas ma) was upregulated at 80 d compared with 66 d after transplanting,and their nicotine content in root and leaves was also increased.The expressions of NtMYC2b,ornithine decarboxylase gene(ODC),arginine de carboxylase gene(ADC) ,putrescine-N-methyltransferase gene (PMT) and N-methylputrescine oxidase gene(MPO) were significantly upregulated in root of hybrid relative to their parents.The relative expres sion levels of NtMYC2b,ODC,ADC,PMT and QPT(quinoline phosphoribosyltransferase gene) in the strong heterosis combination(VA116 × Basma) were significantly higher than those in the weak heterosis combination(VA116 × GDH88) ,meanwhile,the nicotine content and its heterosis also showed the same trend in roots and leaves of hybrid.It indicated that NtMYC2b was involved in the synthesis of nicotine and might also be involved in the formation of heterosis of nicotine content.Correlation analysis showed that the expression of NtMYC2b was significantly positively correlated with PMT in the parent roots,the same with ADC and MPO in hybrid roots.Moreover,between NtMYC2b expression and nicotinic content heterosis,between PMT expression and ODC and ADC expression,was also a significant positive correla tion in hybrid leaves.Sequence analysis showed that the promoter sequence of ADC contained 1 MYC binding site and 1 G-box element,the promoter sequence of PMT contained 7 MYC binding sites and 1 G box element.Therefore,it is speculated that NtMYC2b may regulate the expression of ADC and PMT by specifically binding with cis-element MYC binding site or G-box elementto promote the synthesis of nico tine.These findings suggest that NtMYC2b is involved in the synthesis of nicotine and the formation of het erosis in nicotine content．

Key words： Tobacco; Transcription factors; NtMYC2b; Nicotine; Heterosis

Cite this article

WU Yuyao, XIE Ｒui, YANG Youcheng, LIU Kunhua, NIE Qiong . Transcription Factor NtMYC2b Is Involved in the Formation of Heterosis of Nicotine Content Trait[J]. Journal of Henan Agricultural Sciences, 2019 , 48(11) : 45 -53 . DOI: 10.15933/j.cnki.1004-3268.2019.11.007

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