The homeodomain-leucine zipper protein(HD-Zip) can participate in the hormone signaling pathway by interacting with other proteins,thereby regulating the stress response and growth and develop ment of plants.To verify and explore the response pattern of HD-Zip protein to drought stress and ABA in duction,a HD-Zip transcription factor gene was cloned from maize drought-resistant self-selection line Zheng D58M,which was temporarily named ZmHOX32.The ZmHOX32 protein consisted 856 amino acids and belonged to a hydrophilic protein,which was localized in the nucleus.Phylogenetic tree and conserved motif analysis showed that the ZmHOX32 protein sequence was highly homologous to Setaria italica and Panicum hallii protein sequences,and the conserved motif was completely identical.The ZmHOX32 gene promoter sequence contained binding elements that responded to hormones,abiotic stresses and light stim ulation.The results of qＲT-PCＲ showed that ZmHOX32 gene was a constitutively expressed gene,which was highly expressed in vegetative meristem and was positively induced by drought stress and exogenous ABA.These results indicate that ZmHOX32 gene is involved in drought stress and ABA signaling path way．