&nbsp;Transformation of Phosphorus Efficient Gene OsPHＲ2 into
 Wheat and Tolerance of Transgenic Plants to Low Fertility

HUA Xia, FANG Yuhui, GAO Chong, HAN Liupeng, HU Lin, LI Yan

doi:10.15933/j.cnki.1004-3268.2019.10.002

Journal of Henan Agricultural Sciences >

2019 , Vol. 48 >Issue 10: 6 - 12

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.10.002

Crop Cultivation & Genetic Breeding

Transformation of Phosphorus Efficient Gene OsPHＲ2 into Wheat and Tolerance of Transgenic Plants to Low Fertility

Expand

(Wheat Ｒesearch Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received date: 2019-05-22

Online published: 2019-10-15

Fold

Abstract

Phosphorus is one of the major necessary elements for plant growth and development． In order to obtain transgenic wheat plants with high phosphorus utilization efficiency,OsPHＲ2(Oryza sativa L.phosphate starvation response 2) gene was transferred into Zhengmai 7698 and other five wheat varieties (lines) by particle bombardment transformation method． Genetic analysis was carried out in the positive transgenic wheat progenies.Furthermore，the yield and other traits of T1 transgenic plants under high and low fertility treatments were analyzed.PCＲ results showed that there were 180 positive transgenic wheat plants in T0 generation.Genetic analysis revealed that the OsPHＲ2 gene segregation ratio of 15 lines among 16 lines was in accordance with the law of Mendelian single gene genetic segregation.The results of high and low fertility treatments showed that OsPHＲ2 could significantly increase the wheat yield of sin gle plant under low fertility treatment，but the high fertility treatment was not.The yields of single plant of OsPHＲ2-overexpressing transgenic Zhengmai 1342 and Zhengmai 7698 significantly increased by 11.06% and 9.27% compared with wild type plants under low fertility treatment，which was mainly due to the increase of 1 000-grain weight and grain number per spike．

Key words： Transgenic wheat; OsPHＲ2 gene; High phosphorus efficiency; Low fertility; Yield

Cite this article

HUA Xia, FANG Yuhui, GAO Chong, HAN Liupeng, HU Lin, LI Yan . Transformation of Phosphorus Efficient Gene OsPHＲ2 into Wheat and Tolerance of Transgenic Plants to Low Fertility[J]. Journal of Henan Agricultural Sciences, 2019 , 48(10) : 6 -12 . DOI: 10.15933/j.cnki.1004-3268.2019.10.002

References

［1］胡红青，李学垣，贺纪正.有机酸根与铝氧化物表面吸附磷的解吸［J］.植物营养与肥料学报，1998，4（3）：249-256.

［2］GOLDSTEIN A H.Phosphate starvation inducible enzymes and proteins in higher plants［J］.Society for Experimental Biology Seminar Series，1992，49：25-44.

［3］凌炳琦，柏星轩，周永斌，等.转AtTGA4小麦田间耐低磷胁迫的功能分析［J］. 中国农业科学，2018，51（12）：2225-2234.

［4］RUBIO V.A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae［J］.Genes and Development，2001，15（16）：2122-2133.

［5］LIU F，WANG Z，REN H，et al.OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice ［J］.Plant Journal，2010，62（3）：508-517.

［6］ZHANG Q，WANG C，TIAN J，et al.Identification of rice purple acid phosphatases related to phosphate starvation signalling ［J］.Plant Biology，2011，13（1）：7-15.

［7］TIAN J，WANG C，ZHANG Q，et al.Overexpression of OsPAP10a,a rootassociated acid phosphatase,increased extracellular organic phosphorus utilization in rice ［J］.Journal of Integrative Plant Biology，2012，54（9）：631-639.

［8］ZHOU J，JIAO F C，WU Z C，et al.OsPHR2 is involved in phosphatestarvation signaling and excessive phosphate accumulation in shoots of plants ［J］.Plant Physiology，2008，146（4）：1673-1686.

［9］SECCO D，WANG C，ARPAT B A，et al.The emerging importance of the SPX domaincontaining proteins in phosphate homeostasis ［J］.New Phytologist，2012，193（4）：842-851.

［10］李艳，许为钢，胡琳，等.玉米磷酸烯醇式丙酮酸羧化酶基因高效表达载体构建及其导入小麦的研究［J］. 麦类作物学报，2009，29（5）：741-746.

［11］李艳，许为钢，齐学礼，等.提高小麦基因枪法转化效率的研究［J］.麦类作物学报，2015，35（4）：443-448.

［12］柴建芳，刘旭，贾继增.一种适于PCR扩增的小麦基因组DNA快速提取法［J］.植物遗传资源学报，2006，7（2）：246-248.

［13］PELLEGRINESCHI A，REYNOLDS M，PACHECO M，et al.Stressinduced expression in wheat of the Arabidopsis thaliana DREB1 A gene delays water stress symptoms under greenhouse conditions ［J］.Genome，2004，47（3）：493-500.

［14］栗聪，雒景吾，张磊，等.小麦成熟胚再生体系优化及优良受体基因型筛选［J］.麦类作物学报，2014，34（5）：583-590.

［15］蒋云，张洁，宣朴，等.四川小麦优良受体基因型筛选及农杆菌转化因素优化［J］.分子植物育种，2019，17（4）：1203-1212.

［16］张彦，喻修道，唐克轩，等.用基因枪法获得转异天南星基因aha抗蚜虫小麦［J］.作物学报，2012，38（8）：1538-1543.

［17］李三和，林刚，张金锐，等.转基因小麦外源基因在主栽小麦种质中的遗传规律［J］.麦类作物学报，2005， 25（5）：23-26.

［18］KIM W，AHN H J，CHIOU T J，et al.The role of the miR399PHO2 module in the regulation of flowering time in response to different ambient temperatures in Arabidopsis thaliana［J］.Molecules and Cells，2011，32（1）：83-88.

［19］LIU T Y，HUANG T K，TSENG C Y，et al.PHO2dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis［J］.The Plant Cell，2012，24（5）：2168-2183.

［20］刘翠.转录因子GmPHR1和OsPHR2转化大豆及其转基因新材料创制［D］.保定：河北农业大学，2012.

［21］WU P，WANG Z Y.Molecular mechanisms regulating Pisignaling and Pi homeostasis under OsPHR2,a central Pisignaling regulator in rice［J］.Frontiers in Biology，2011，6（3）：242-245.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References