Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides
 Genes to Population Density in Bean Beetle

CHEN Qianquan, WANG Yeying

doi:10.15933/j.cnki.1004-3268.2019.09.013

Journal of Henan Agricultural Sciences >

2019 , Vol. 48 >Issue 9: 90 - 97

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.09.013

Plant Protection

Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides Genes to Population Density in Bean Beetle

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(School of Life Sciences，Guizhou Normal University，Guian 550025，China)

Received date: 2019-04-09

Online published: 2019-09-15

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Abstract

In order to unveil the response mechanism of genes related to immunity signal transduction and antimicrobial peptide to population density，tBLASTn was used to identify genes related to Toll，IMD(Im mune deficiency) and antimicrobial peptide genes from ＲNA-sequencing data of bean beetle(Callosobru chus maculatus) .FPKM(Fragments per kilobase per million mapped reads) value was used to measure the expression level of these genes.The results showed that six Toll signaling pathway receptor genes which encoded proteins containing leucine-rich repeats domains were identified.Toll-1 had the fastest evo lutionary rate，while the evolutionary rates of Toll-3，Toll-10，Toll-7，Toll-8 and Toll-6 decreased in turn．IMD，an intracellular key signal gene，and Peptidoglycan recognition protein LC(PGＲP-LC) ，a receptor gene of IMD signaling pathway，were identified． IMD and PGＲP-LC had similar evolutionary rate to Toll 3.At expression level，Toll-1 was the lowest(FPKM=0.32) ，and was not regulated by population densi ty.The expression levels of Toll-3，Toll-6，Toll-7，Toll-8，and Toll-10 were significantly or extremely signif icantly up-regulated in high-density individuals，with Toll-7 having the highest expression level(its FP KMs in high-density individuals and low-density individuals were 127.25 and 57.92，respectively) ． Popu lation density had no significant effect on the expression level of IMD and PGＲP-LC.Seven antibacterial peptide genes were identified，including one Defensin(defensin gene) ，one Cecropin(cetanin gene) ，two Apidaecins(bee antibacterial peptide genes) ，and three Drosomycins(anti-fungal peptide genes) .Among them，Drosomycin was the most conservative one in evolution.Antibacterial peptide Apidaecin-1 had the lowest expression level(FPKM=6.58) and Defensin had the highest expression level(its FPKMs in high density individuals and low-density individuals were 1 277.23 and 683.87，respectively) .Population den sity had no significant effect on the expression level of Apidaecin-1，Cecropin and Apidaecin-2.Defensin and three Drosomycin genes were up-regulated in high density individuals.In summary，Toll-3，Toll-6， Toll-7，Toll-8，Toll-10，Defensin，Drosomycin-1，Drosomycin-2 and Drosomycin-3 were up-regulated under high population density．

Cite this article

CHEN Qianquan, WANG Yeying . Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides Genes to Population Density in Bean Beetle[J]. Journal of Henan Agricultural Sciences, 2019 , 48(9) : 90 -97 . DOI: 10.15933/j.cnki.1004-3268.2019.09.013

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