Plant Protection

 Cloning and Expression Analysis of PvARP1 Gene Encoding Auxin-regulating Protein in Common Bean

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  •  (Crop Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

Received date: 2019-03-15

  Online published: 2019-09-15

Abstract

 Auxin-regulating protein (ARP) is an important factor involved in the regulation of plant growth and development,and is also a protein molecule that plays an important role in plant disease resist ance. In order to understand the gene expression characteristics of this protein,the cDNA sequence enco ding the ARP protein was cloned and analyzed by using the expression sequence tag(EST) of common bean(Phaseolus vulgaris L.) .Sequence analysis indicated that the cDNA fragment was 2 428 bp in length and named as PvARP1.It contained an open reading frame(ORF) of 1 818 bp,and the accession number was MK301448.The gene encoded 605 amino acids and the predicted protein molecular weight was 68.26 ku.The encoded protein did not contain transmembrane region and signal peptide.The results of homolo gous analysis showed that the close relationship between PvARP1 protein and ARP protein of Vigna angu  laris reached 94%.eal time PCR analysis showed that PvARP1 gene was regulated by Fusarium oxyspo rum f.sp.phaseoli FOP-DM01 isolate.The expression of PvARP1 gene at 24 h after inoculation was in creased to 111 times of that at 0 h as control in the roots of the resistant material 260205 to the disease, and the expression level in 260205 roots was higher than the susceptible material BRB130 at all time points.The expression of PvARP1 gene was significantly enhanced by indole-3-acetic acid(IAA) .In addi tion,the expression level in the flowers and pods was obviously higher than the roots,stems and leaves.The PvARP1 gene could be expressed as a 68 ku protein in E.coli BL21(DE3) ,which had the amide synthetase activity in vitro,and might regulate the active IAA level in plant cells.This study indicated that the PvARP1 gene probably participated in the defense response of common bean to the FOP-DM01 isolate through the IAA-mediated signaling pathway.It is speculated that the PvARP1 gene is related to the resist ance of fusarium wilt in common bean,and has the great significance for directing the molecular breeding of resistance in common bean.

Cite this article

XUE Renfeng, FENG Ming, ZHAO Yang, CHEN Jian, LI Tao, GE Weide .  Cloning and Expression Analysis of PvARP1 Gene Encoding Auxin-regulating Protein in Common Bean[J]. Journal of Henan Agricultural Sciences, 2019 , 48(9) : 82 -89 . DOI: 10.15933/j.cnki.1004-3268.2019.09.012

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