Crop Cultivation & Genetic Breeding

 Characterization of Nitric Oxide Sensitive Mutant rsn1 in Arabidopsis

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  •  (Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China)

Received date: 2019-05-22

  Online published: 2019-09-15

Abstract

 In order to investigate the molecular regulation mechanism of nitric oxide(NO) in plant,using Arabidopsis as research materials,we screened NO-sensitive mutants and isolated a mutant rsn1 (root sensitive to NO) from the EMS mutant library. The sensitivity of rsn1 mutant roots to NO and stomatal response to abscisic acid(ABA) were compared and analyzed,and the mutant genes were preliminarily located by map-based cloning.The results were as follows: the rsn1 root was more sensitive to NO stress compared with the wild type,though the germination rate of rsn1 mutant was not significantly different from that of wild type under normal growth conditions.Under 50 μmol/L SNP treatment,the relative root length of wild type( as compared with root length in normal growth) was 48.7%,whereas the relative root length of mutant was 5.3%.By analyzing the sensitivity of stomata to ABA,it was found that rsn1 was insensitive to the regulation of stomatal closure by ABA.The stomatal diameter of rsn1 mutant was 1.9 times that of wild type under 10 μmol/L ABA treatment,though the stomatal diameter of rsn1 mutant was similar to that of wild type under normal conditions,and the water loss rate of rsn1 mutant was higher than that of wild type Arabidopsis.Genetic analysis suggested that rsn1 was a single recessive mutant,and the mutation site was initially designated on chromosome 5 by map-based cloning.

Cite this article

XIA Jinchan, CONG Renyuan, ZHANG Xiaoli .  Characterization of Nitric Oxide Sensitive Mutant rsn1 in Arabidopsis[J]. Journal of Henan Agricultural Sciences, 2019 , 48(9) : 40 -45 . DOI: 10.15933/j.cnki.1004-3268.2019.09.006

References

1HUANG T  F,JANDER G.Abscisic acidregulated protein degradation causes osmotic  stressinduced accumulation of branchedchain amino acids in Arabidopsis thaliana[J.Planta,2017,246(4):737-747.

2MA Y,SZOSTKIEWICZ I,KORTE A,et al.Regulators of PP2C  phosphatase activity function as abscisic acid sensorsJ.Science,2009,324(5930):1064-1068.

3PARK S Y,FUNG P,NISHIMURA N,et al.Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteinsJ.Science,2009,324(5930):1068-1071.

4SCHWEIGHOFER A,HIRT H,MESKIENE I.Plant PP2C phosphatases:Emerging functions in stress signalingJ.Trends in Plant Science,2004,9(5):236-243.

5FUJITA Y,NAKASHIMA K,YOSHIDA T,et al.Three SnRK2 protein   kinases are the main positive regulators of abscisic acid signaling   in response to water stress in Arabidopsis[J.Plant and  Cell Physiololgy,2009,50(12):2123-2132.

6WANG J L,ZHANG Q,YU Q,et al.CARK6 is involved in abscisic acid to regulate stress responses in Arabidopsis thaliana[J.Biochem Biophys Res Commun,2019,513(2):460-464.

7CORPAS F J,BARROSO J B,CARRERAS A,et al.Constitutive argininedependent nitric oxide synthase activity in   different organs of pea seedlings during plant developmentJ.Planta,2006,224(2):246-254.

8ZHAO Z G,CHEN G C,ZHANG C L.Interaction between reactive oxygen species and nitric oxide in droughtinduced abscisic acid synthesis in root tips of wheat seedlingsJ.Aust J Plant Physiol,2001,28(10):1055-1061.

9LESHEM Y Y,HARAMATY E.Plant aging:The emission of NO and ethylene and the effect of NOreleasing compounds on growth of pea(Pisum sativum) foliageJ.J Plant Physiol,1996,148:258-263.

10ALLEN G J,CHU S P,HARRINGTON C L,et al.A defined range of guard cell calcium oscillation parametersencodes stomatal movementsJ.Nature,2001,411(6841):1053-1057.

11LEE Y,CHOI Y B,SUH S,et al.Abscisic acidinduced phosphoinositide turnover in guard cellprotoplasts of Vicia fabaJ.Plant Physiol,1996,110(3):987-996.

12MCAINSH M R,BROWNLEE C,HETHERINGTON A M.Abscisic acidinduced elevation of  guard cell cytosolic Ca2+precedes stomatal closureJ.Nature,1990,343(6254):186-188.

13WANG P C,DU Y Y,HOU Y J,et al.Nitric oxide negatively regulates abscisic acid signaling in guard cells by Snitrosylation of OST1J.Proc Natl Acad Sci USA,2015,112(2):613-618.

14DESIKAN R,GRIFFITHS R,HANCOCK J,et al.A new role for an old enzyme:Nitratereductasemediated nitric oxide generation is required for abscisic acidinduced stomatal  closure in Arabidopsis thaliana[J.Proc Natl Acad Sci USA,2002,99(25):16314-16318.

15LOZANOJUSTE J,LEN J.Enhanced abscisic acidmediated responses in nia1nia2noa12 triple mutant impaired in NIA/NR and AtNOA1dependent nitric oxide   biosynthesis in Arabidopsis[J.Plant Physiol,2010,152(2):891903.

16SONG C P,AGARWAL M,OHTA M,et al.Role of an Arabidopsis AP2/EREBPtype transcriptional repressor in abscisic acid and drought stress responsesJ.Plant Cell,2005,17(8):2384-2396.

17LUKOWITZ W,GILLMOR C S,SCHEIBLE W R.Positional cloning in Arabidopsis.Why it feels good to have a genome initiative working for youJ.Plant Physiology,2000,123(3):795-805.

18HE Y K,TANG R H,HAO Y,et al.Nitric oxide represses the Arabidopsisfloral transitionJ.Science,2004,305(5692):1968-1971.

19SIDDIQUI M H,ALWHAIBI M H,BASALAH M O.Role of nitric oxide in tolerance of plants to abiotic stressJ.Protoplasma,2011,248(3):447-455.

20FANCY N N,BAHLMANN A K,LOAKE G J.Nitric oxide function in plant abiotic stressJ.Plant Cell  and Environment,2017,40(4):462-472.

21FAN Q J,LIU J H.Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal responseJ.Plant Cell Reports,2012,31(1):145-154.

22KALE L,NAKURTE I,JALAKAS P,et al.Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductanceJ.Plant Physiol Biochem,2019,140:18-26.

23NEILL S J,DESIKAN R,CLARKE A,et al.Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cellsJ.Plant Physiol,2002,128(1):13-16.

24GARCIAMATA C,GAY R,SOKOLOVSKI S,et al.Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acidevoked signaling pathwaysJ.Proc Natl Acad Sci USA,2003,100(19):11116-11121.

25VAHISALU T,KOLLIST H,WANG Y F,et al.SLAC1 is required for plant guard cell Stype anion channel function in stomatal signallingJ.Nature,2008,452(7186):487-491.

26JOUDOI T,SHICHIRI Y,KAMIZONO N,et al.Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis[J.Plant Cell,2013,25(2):558-571.

27]杨凤博,王鲜萍,李坤.拟南芥蛋白激酶SNRK2.6的原核表达、纯化及活性分析[J.河南农业科学,2014,43(10):26-29.

28]雷凯健,王棚涛,刘浩.拟南芥响应过氧化氢突变体的筛选及遗传分析[J.河南农业科学,2015,44(10):53-56.

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