Identification of Biocontrol Strain YB‐366 and Its Control Effect against Plant‐Parasitic Nematodes

doi:10.15933/j.cnki.1004-3268.2025.06.010

Abstract

Abstract: Plant‐parasitic nematodes severely threaten global agricultural production security.To explore superior biocontrol strains against plant‐parasitic nematodes，this study evaluated the biocontrol potential of a nematophagous fungal strain YB‐366 against wheat cyst nematode disease（Heterodera filipjevi）and vegetable root‐knot nematode disease（Meloidogyne incognita）. Firstly，the in vitro determination method was used to assess the lethal effects of YB‐366 fermentation filtrate on second‐stage juveniles of H.filipjevi and M.incognita，as well as its inhibition on egg hatching. Further pot experiments and field trials were conducted to verify the control efficacy of YB‐366 against these two nematode diseases.Morphological characterization and molecular identification were subsequently performed to determine the taxonomic status of strain YB‐366. Results showed that the fermentation filtrate caused corrected mortality rates of 95.6% and 93.5% for H.filipjevi and M.incognita J2 larvae within 48 h，respectively，and significantly inhibited egg hatching with suppression rates of 81.6% and 78.5%.In indoor and field trials，soil treatment with YB‐366 wheat bran‐sand culture reduced cyst number in wheat by 60.5% and 55.2%，respectively，and achieved control efficiencies of 60.7% and 58.9% against vegetable root‐knot nematodes. Additionally，the treatment markedly promoted the growth of wheat and tomato plants.Based on morphological and molecular identification，YB‐366 was classified as Chaetomium megalocarpum.Overall，C.megalocarpum strain YB‐366 demonstrates substantial potential for development and utilization in biocontrol agent research and formulation development，as well as the establishment of green pest management systems for sustainable agriculture.

Key words: Chaetomium megalocarpum, Heteradera filipjevi, Meloidogyne incognita, Biological control

摘要： 植物寄生线虫在全球范围内对农作物的生产安全构成严重威胁。为挖掘优良的植物寄生线虫生防菌株，对分离到的一株线虫生防真菌YB-366进行小麦孢囊线虫病和果蔬根结线虫病的生防潜力评估。首先通过离体测定方法测定YB-366菌株发酵滤液对菲利普孢囊线虫（Heteradera filipjevi）和南方根结线虫（Meloidogyne incognita）二龄幼虫的致死作用及其卵孵化的抑制作用，进一步利用盆栽试验和田间试验验证YB-366菌株对小麦孢囊线虫病和果蔬根结线虫病的防治效果，并通过形态学和分子生物学方法对YB-366菌株进行鉴定。结果显示，YB-366菌株发酵滤液对菲利普孢囊线虫和南方根结线虫的二龄幼虫均表现出较强的致死作用，48 h校正死亡率分别高达95.6%、93.5%，且有效抑制了2种线虫卵的孵化，孵化抑制率分别为81.6%、78.5%。室内和田间防效试验中，YB-366麦粒砂培养物土壤处理对小麦孢囊线虫病的孢囊减退率分别为60.5%和55.2%，对果蔬根结线虫病的防治效果分别为60.7%和58.9%，同时显著促进了小麦和番茄植株的生长。通过形态学和分子生物学方法将YB-366鉴定为大果毛壳菌（Chaetomium megalocarpum）。综上所述，大果毛壳菌YB-366菌株在生物防治制剂研发、绿色防控体系构建中具有较好的开发利用潜力。

关键词: 大果毛壳菌, 菲利普孢囊线虫, 南方根结线虫, 生物防治

CLC Number:

S476⁺.15

CHAI Qiuyuan, ZHANG Jie, LI Xiaoqiang, SONG Yajing, XIA Mingcong, SUN Runhong, XU Wen, WU Chao, YANG Lirong. Identification of Biocontrol Strain YB‐366 and Its Control Effect against Plant‐Parasitic Nematodes[J]. Journal of Henan Agricultural Sciences, 2025, 54(6): 92-99.

柴秋源, 张洁, 李晓强, 宋雅静, 夏明聪, 孙润红, 徐文, 武超, 杨丽荣. 植物寄生线虫生防菌株YB-366 的种类鉴定及防效测定[J]. 河南农业科学, 2025, 54(6): 92-99.

Figures/Tables 7

References

［1］PARRADO L M，QUINTANILLA M. Plant‐parasitic nematode disease complexes as overlooked challenges to crop production［J］. Frontiers in Plant Science，2024，15：1439951.

［2］SAMANTA S，SHELAR V B，BANERJEE S，et al.Effects of disease complex of penicellium and root knot nematode on the growth and development of chilli（Capsicum annum L.）crop［J］. Journal of Advances in Biology & Biotechnology，2024，26（12）：42‐50.

［3］VENNAM S. Editorial note on nematode diseases of plants［J］.Journal of Plant Pathology & Microbiology，2021，12（10）：1.

［4］吴秀庆，李彩斌，罗杰，等. 贵州烟草根结线虫种类鉴定及其与根茎类病害混合侵染情况调查［J］. 南方农业学报，2024，55（10）：3013‐3022.

WU X Q，LI C B，LUO J，et al. Identification of tobacco root‐knot nematode species and survey on their mixed infestations with root and tuber diseases in Guizhou［J］.Journal of Southern Agriculture， 2024， 55 （10）：3013‐3022.

［5］SMANT G， HELDER J， GOVERSE A. Parallel adaptations and common host cell responses enabling feeding of obligate and facultative plant parasitic nematodes［J］. The Plant Journal，2018，93（4）：686‐702.

［6］曲硕，刘芳，宋庚晨，等. 大豆抗胞囊线虫锌指蛋白基因GmC₂H₂‑2like克隆与初步功能分析［J］.华北农学报，2024，39（3）：195‐207.

QU S，LIU F，SONG G C，et al. Soybean cyst nematode resistant zinc finger protein gene GmC₂H₂-2like cloning and preliminary functional analysis［J］. Acta Agriculturae Boreali‐Sinica，2024，39（3）：195‐207.

［7］刘荣荣，王暄，李红梅，等. 河南省小麦主产区菲利普孢囊线虫与禾谷孢囊线虫发生情况调查［J］.植物保护，2017，43（5）：157‐163.

LIU R R，WANG X，LI H M，et al. Survey of the occurrence of Heterodera filipjevi and H. avenae on wheat in main growing area in Henan Province［J］.Plant Protection，2017，43（5）：157‐163.

［8］SMILEY R W，DABABAT A A，IQBAL S，et al. Cereal cyst nematodes：A complex and destructive group of Heterodera species［J］. Plant Disease，2017，101（10）：1692‐1720.

［9］赵洪海，刘维志，梁晨，等. 根结线虫在中国的一新纪录种：拟禾本科根结线虫Meloidogyne graminicol［J］. 植物病理学报，2001，31（2）：184‐188.

ZHAO H H，LIU W Z，LIANG C，et al. Meloidogyne graminicola，a new record species from China［J］. Acta Phytopathologica Sinica，2001，31（2）：184‐188.

［10］徐姣，刘佳，代君丽，等.中国黄淮麦区菲利普孢囊线虫淮阳和博爱致病型群体特异性SCAR标记的建立［J］.麦类作物学报，2016，36（4）：523‐530.

XU J，LIU J，DAI J L，et al. Development of SCAR marker for detecting Heterodera filipjevi of Huaiyang and Boai pathotype populations from wheat‐growing regions of the Huang‐Huai‐Hai Plain in China［J］.Journal of Triticeae Crops，2016，36（4）：523‐530.

［11］邓小大，袁永强，蔡书静，等. 基于RNA-seq的番茄响应根结线虫MiPDCD6蛋白的SA信号通路基因鉴定与表达分析［J］.华北农学报，2023，38（1）：184‐195.

DENG X D，YUAN Y Q，CAI S J，et al. Identification and expression analysis of tomato SA signaling pathway genes in response to root knot nematode MiPDCD6 protein based on RNA‐seq［J］. Acta Agriculturae Boreali‐Sinica，2023，38（1）：184‐195.

［12］刘雅洁，罗书介，蒋晓惠，等. 两株芽胞杆菌的分离鉴定及其对根结线虫病的防治效果［J］. 植物病理学报，2024，54（3）：625‐637.

LIU Y J，LUO S J，JIANG X H，et al. Identification and biocontrol effect of two Bacillus strains against root‐knot disease［J］. Acta Phytopathologica Sinica，2024，54（3）：625‐637.

［13］LI Y，LU Q S，WANG S，et al. Discovery of a root‐lesion nematode，Pratylenchus scribneri，infecting corn in Inner Mongolia，China［J］. Plant Disease，2019，103（7）：1792.

［14］AKHTER G，ZAFAR A，KHAN W，et al. In vitro nemato‐toxic potential of some leaf extracts on Juvenile mortality of Meloidogyne incognita race‐3［J］.Archives of Phytopathology and Plant Protection，2018，51（7/8）：399‐407.

［15］彭静文，赵远征，王真，等. 防治马铃薯腐烂茎线虫病的植物源药剂筛选［J］.河南农业科学，2022，51（4）：95‐102.
PENG J W，ZHAO Y Z，WANG Z，et al.Screening of botanical pesticides for controlling Ditylenchus destructor［J］.Journal of Henan Agricultural Sciences，2022，51（4）：95‐102.

［16］王广印，郭卫丽，陈碧华，等.RSD防控设施土壤和蔬菜连作障碍机制及应用的研究进展［J］.河南农业科学，2023，52（1）：1‐10.
WANG G Y，GUO W L，CHEN B H，et al.Research progress on mechanism and application of reductive soil disinfection（RSD）in prevention and control of continuous cropping obstacles of facility soil and vegetable［J］.Journal of Henan Agricultural Sciences，2023，52（1）：1‐10.

［17］彭宇龙，孙小芳，黄云，等. 纳米ZnO在植物病害防控中的应用研究进展［J］.河南农业科学，2025，54（2）：1‐9.
PENG Y L，SUN X F，HUANG Y，et al. Research progress on nano‐ZnO applications in plant disease control［J］.Journal of Henan Agricultural Sciences，2025，54（2）：1‐9.

［18］刘勇鹏，张涛，王秋岭，等. 生物菌剂防治设施蔬菜根结线虫研究进展［J］.中国瓜菜，2020，33（10）：9‐14.
LIU Y P，ZHANG T，WANG Q L，et al.Research progress of biocontrol agents against root knot nematodes in protected vegetable production［J］.China Cucurbits and Vegetables，2020，33（10）：9‐14.

［19］徐雪亮，范琳娟，吴彩云，等. 生防微生物对植物病原线虫主要作用机制概述［J］.南方农业学报，2023，54（10）：2969‐2977.
XU X L，FAN L J，WU C Y，et al. Main action mechanism of biocontrol microorganisms on plant pathogenic nematodes：A review［J］. Journal of Southern Agriculture，2023，54（10）：2969‐2977.

［20］ALI A A I，MAHGOUB S A，AHMED A F，et al.Utilizing endophytic plant growth‑promoting bacteria and the nematophagous fungus Purpureocillium lilacinum as biocontrol agents against the root‑knot nematode（Meloidogyne incognita） on tomato plants ［J］. European Journal of Plant Pathology，2024，170 （2）：417‑436.

［21］LUBIAN C，MATINHA D D，PORTZ R L，et al.Hirsutella thompsonii and Pochonia chlamydosporia（Syn.Verticillium chlamydosporium） mycelia growth and predation on Panagrellus redivivus［J］. Journal of Agricultural Science，2017，9（11）：137.

［22］段玉玺，靳莹莹，王胜君，等. 生防菌株Snef85的鉴定及其发酵液对不同种类线虫的毒力［J］.植物保护学报，2008，35（2）：132‑136.
DUAN Y X，JIN Y Y，WANG S J，et al. Identification of biocontrol strain Snef85 and virulence of fermented broth on different nematodes［J］.Journal of Plant Protection，2008，35（2）：132‑136.

［23］张洁，袁虹霞，孙炳剑，等. 小麦孢囊线虫病生防真菌08F04菌株的鉴定及防效测定［J］.中国生物防治学报，2013，29（4）：509‑514.
ZHANG J，YUAN H X，SUN B J，et al. Identification and effects of biocontrol fungal strain 08F04 on cereal cyst nematode of wheat［J］.Chinese Journal of Biological Control，2013，29（4）：509‑514.

［24］ZHANG J，LI Y H，YUAN H X，et al. Biological control of the cereal cyst nematode（Heterodera filipjevi）by Achromobacter xylosoxidans isolate 09X01 and Bacillus cereus isolate 09B18［J］. Biological Control，2016，92：1‑6.

［25］YAO Y R，TIAN X L，SHEN B M，et al. Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita［J］. World Journal of Microbiology &Biotechnology，2015，31（4）：549‑556.

［26］ZHANG J，WANG L M，LI Y H，et al. Biocontrol of cereal cyst nematode by Streptomyces anulatus isolate S07［J］. Australasian Plant Pathology，2016，45（1）：57‑64.

［27］SUN M H，GAO L，SHI Y X，et al. Fungi and actinomycetes associated with Meloidogyne spp.eggs and females in China and their biocontrol potential［J］.Journal of Invertebrate Pathology，2006，93（1）：22‑28.

［28］刘杏忠，张克勤，李天飞. 植物寄生线虫生物防治［M］. 北京：中国科学技术出版社，2004.
LIU X Z，ZHANG K Q，LI T F. Biological control of plant‑parasitic nematodes［M］.Beijing：China Science and Technology Press，2004.

［29］GARABEDIAN S，VAN GUNDY S D. Use of avermectins for the control of Meloidogyne incognita on tomatoes［J］.Journal of Nematology，1983，15（4）：503‑510.

［30］魏景超. 真菌鉴定手册［M］. 上海：上海科学技术出版社，1979.
WEI J C. Fungal identification handbook［M］.Shanghai：Shanghai Scientific & Technical Publishers，1979.

［31］鄢小宁. 利用黄瓜幼苗内生真菌种子处理防治南方根结线虫的研究［D］. 杭州：浙江大学，2010.
YAN X N. Study on prevention and control of root‑knot nematodes in Southern China by seed treatment of endophytic fungi in cucumber seedlings［D］. Hangzhou：Zhejiang University，2010.

［32］BAIRWA A，DIPTA B，MHATRE P H，et al. Chaetomium globosum KPC3：An antagonistic fungus against the potato cyst nematode， Globodera rostochiensis［J］. Current Microbiology， 2023， 80 （4）：125.

［33］冯超红，李丽娟，张姣姣，等.球毛壳菌促生防病机制及应用研究进展［J］. 中国生物防治学报，2023，39（4）：961‑969.
FENG C H，LI L J，ZHANG J J，et al. Mechanism of Chaetomium globosum in promoting growth and preventing disease and advance of its application and research［J］. Chinese Journal of Biological Control，2023，39（4）：961‑969.

［34］李月，胡德升，谭金芳，等. 单列毛壳菌通过促进秸秆降解并调控激素响应基因表达促进玉米生长［J］.植物学报，2022，57（4）：422‑433.
LI Y，HU D S，TAN J F，et al.Chaetomium uniseriatum promotes maize growth by accelerating straw degradation and regulating the expression of hormone responsive genes［J］.Chinese Bulletin of Botany，2022，57（4）：422‑433.

［35］NITAO J，MEYER S，CHITWOOD D，et al. Isolation of flavipin，a fungus compound antagonistic to plant‑parasitic nematodes［J］. Nematology，2002，4（1）：55‑63.

［36］HU Y，ZHANG W P，ZHANG P，et al. Nematicidal activity of chaetoglobosin A poduced by Chaetomium globosum NK102 against Meloidogyne incognita［J］.Journal of Agricultural and Food Chemistry，2013，61 （1）：41‑46.

[1]	ZHU Lulu, LIU Jinli, LU dan, CHAI Chunyue. Taxonomic Identification of the Yeast Strain 4⁃41 and Characterization of the Antagonistic Mechanisms against Botrytis cinerea [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 89-99.
[2]	ZHANG Qiang, WU Limin, LI Pengyan, ZHAO Na, HU Shen’ao, LU Ninghai. Optimization of Fermentation Conditions and Stability Evaluation of Antagonistic Bacteria against Wheat Crown Rot [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 121-129.
[3]	LIANG Song, MENG Xiangkun, YU Xin, XU Wenfeng, XING Fangfang, ZHENG Shulin, LI Xinzhu, WANG Liang. Isolation and Identification of Bacillus for Biocontrol of Leaf Blight of Strawberry and Study on Its Biocontrol Effect [J]. Journal of Henan Agricultural Sciences, 2023, 52(10): 92-99.
[4]	WANG Yaxin, YAN Fei, LI Jianlong. Effects of Three Antagonistic Yeasts Treatment on the Storage Quality of Honey Peach [J]. Journal of Henan Agricultural Sciences, 2022, 51(7): 173-180.
[5]	ZHANG Yuqi, REN Yueying, WEN Xiangsui, WANG Yumeng, FANG Ping, LI Baoquan, LENG Shihe. Screening and Control Effect of Endophytic Fungi for Brown Spot Disease of Schisandra chinensis [J]. Journal of Henan Agricultural Sciences, 2021, 50(8): 94-102.
[6]	ZHANG Hongyan, ZHANG Jingjing, WANG Shanshan, WANG Yaling, WANG Qingqing, WANG Xiangping. Parasitic Efficacy and Development Performance of Cotesia marginiventris(Cresson) on Spodoptera frugiperda(Smith) [J]. Journal of Henan Agricultural Sciences, 2021, 50(5): 86-91.
[7]	LIU Lu, WAN Weijie, ZHENG Tongwen, LONG Xinyu, SUN Zhengxiang, ZHOU Yi. Control Effect of Burkholderia pyrrocinia YZU-S377 on Cotton Verticillium Wilt and Its Growth Promotion Function [J]. Journal of Henan Agricultural Sciences, 2021, 50(3): 91-96.
[8]	ZHU Zhiyan, CHEN Minghua, CHEN Qiang, TIAN Zhihong, LI Jianxiong. Control Effect of Streptomyces Sm4‐1986 on Rice Sheath Blight [J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 92-102.
[9]	XIA Mingcong, DENG Xiaoxu, QI Hongzhi, XIE Xia, XU Wen, ZHANG Jie, SUN Runhong, PAN Yamei, WU Chao, YANG Lirong. Biological Control of Sharp Eyespot and Growth Promotion in Wheat by Bacillus velezensis YB‑145 [J]. Journal of Henan Agricultural Sciences, 2021, 50(10): 76-83.
[10]	LI Wenpeng, TAO Ye, ZHAO Suya, NIU Qiuhong. Antagonism of Bacillus sp.HT-7 against Verticillium wilt of Cotton and Exploration of Antagonistic Factors [J]. Journal of Henan Agricultural Sciences, 2020, 49(8): 84-93.