Research on the Occurrence in Fields and Detection of Tobacco Viruses in Henan Province

doi:10.15933/j.cnki.1004-3268.2022.12.011

Abstract

Abstract: Tobacco virus disease is an important disease affecting tobacco growth，which occurs periodically and regionally.To clarify the occurrence in fields and variation of tobacco viruses in Henan Province in recent years，we systematically investigated the dynamics of tobacco viruses in the southern，central and western regions of Henan respectively，and detected tobacco viruses samples by ELISA simultaneously. The results indicated that the dynamic curve of tobacco virus disease in field was S‑shaped，with a initial stage appearing at 25—30 days after transplanting，then a peak emerging after 60—70 days，and at last reaching a stable stage when plants were ripe.It also showed that the main virus species were tobacco mosaic virus（TMV），cucumber mosaic virus（CMV），tobacco etch virus（TEV），tobacco vein banding mosaic virus（TVBMV）and potato virus Y（PVY）from 2017 to 2021.

Key words: Tobacco virus disease, Henan tobacco?growing area, Occurrence in fields, Species analysis, ELISA detection

摘要： 烟草病毒病是影响烟草生长的一种重要病害，其发生具有周期性、区域性等特点。为明确河南烟区近年来病毒病田间发生动态和主要病毒种类变化，采用系统调查方法调查豫南、豫中、豫西烟区病毒病田间发生动态，并利用酶联免疫吸附法检测采自豫南、豫中、豫西烟区的病毒病样本。结果显示，烟草病毒病的田间发生动态曲线呈S形，初发期是在烟草移栽后的25~30 d，60~70 d后达到高峰期，烟株成熟后进入稳定期。研究结果还显示，2017—2021 年河南烟区主要病毒种类是烟草普通花叶病毒（Tobacco mosaic virus，TMV）、黄瓜花叶病毒（Cucumber mosaic virus，CMV）、烟草蚀纹病毒（Tobacco etchvirus，TEV）、烟草脉带花叶病毒（Tobacco vein banding mosaic virus，TVBMV）和马铃薯Y 病毒（Potato virus Y，PVY）。

关键词: 烟草病毒病, 河南烟区, 田间发生动态, 种类分析, ELISA检测

CLC Number:

S435

BAI Jingke, WU Yanhui, NIU Longlong, HAO Haohao, WANG Xuefen, ZHANG Xiaoli, LI Shujun. Research on the Occurrence in Fields and Detection of Tobacco Viruses in Henan Province[J]. Journal of Henan Agricultural Sciences, 2022, 51(12): 90-96.

白静科, 吴彦辉, 牛龙龙, 郝浩浩, 王雪芬, 张晓丽, 李淑君. 河南烟草病毒病田间发生动态及种类监测分析[J]. 河南农业科学, 2022, 51(12): 90-96.

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