Abstract: To provide a theoretical basis for standardized cultivation, the effects of two kinds of tree shape,“厂”-shape and fan-shape on sugar metabolism and accumulation in wine grape (Cabernet Sauvignon, Chardonnay) were studied.The contents of glucose, fructose and sucrose in grapes were determined by ion chromatography. The total sugar content and the activities of acid invertase(AI),neutral invertase(NI),sucrose synthase(SS) and sucrose phosphate synthase(SPS) were determined by physiological and biochemical methods.The results showed that the“厂”-shaped tree shape was beneficial to the accumulation of glucose,fructose and sucrose in‘Cabernet Sauvignon’ grape,fructose and sucrose in‘Chardonnay’grape,and fan-shaped tree shape was beneficial to glucose accumulation in‘Chardonnay’ grape.However,the total sugar contents of ‘Cabernet Sauvignon’ and ‘Chardonnay’grape were higher in the shape of fan than in the shape of “厂”.Tree shape had significant influence on AI and SPS activities of ‘Cabernet Sauvignon’ grape,and the difference reached significant level at the color transformation stage and mature stage. The AI activity of the“厂”-shaped tree shape was higher than that of the fan-shaped tree shape,and activities of NI,SS and SPS in the shape of fan were better than that in the shape of “厂”.The activities of AI,NI,SS and SPS of ‘Chardonnay’grape in the fanshape were better than those in the shape of “厂”.The tree shape of fan was more beneficial to the accumulation of total sugar and the increase of enzyme activity in wine grape.The tree shape of “厂”was more beneficial to the accumulation of glucose,fructose,and sucrose in‘Cabernet Sauvignon’,and the accumulation of fructose and sucrose in ‘Chardonnay’.

Key words: Tree shape, Wine grape, Cabernet Sauvignon, Chardonnay, Total sugar, Monosaccharide;Sucrose metabolism enzyme

摘要： 研究厂字形和扇形2种树形对酿酒葡萄（赤霞珠、霞多丽）果实糖代谢与积累的影响，为其标准化栽培提供理论基础。采用离子色谱法测定葡萄果实中葡萄糖、果糖、蔗糖3种单糖的含量；采用生理生化方法测定总糖含量以及酸性转化酶（AI）、中性转化酶（NI）、蔗糖合成酶（SS）、蔗糖磷酸合成酶（SPS）活性。结果表明，厂字形树形有利于赤霞珠葡萄糖、果糖、蔗糖的积累和霞多丽果糖、蔗糖的积累，扇形树形有利于霞多丽果实中葡萄糖的积累；但赤霞珠和霞多丽果实成熟期总糖含量都表现为扇形树形高于厂字形树形。树形对赤霞珠葡萄AI和SPS活性具有明显影响，且在转色期和成熟期差异达显著水平，厂字形树形中AI活性明显高于扇形，而扇形树形的NI、SS、SPS活性优于厂字形。扇形树形霞多丽葡萄中AI、NI、SS、SPS活性皆优于厂字形。扇形树形有利于酿酒葡萄总糖的积累和酶活性的升高，而厂字形树形有利于赤霞珠葡萄糖、果糖、蔗糖的积累和霞多丽果糖、蔗糖的积累。

关键词: 树形；酿酒葡萄, 赤霞珠；霞多丽；总糖；单糖；蔗糖代谢酶