Abstract: The bioinformation methods were used to identify the Dof(DNA binding with one finger)transcription factor at genome-wide level in wheat,and the conserved domains,phylogenetic relationship,collinearity and expression patterns were also analyzed,so as to lay the foundation for function study of Dof transcription factor. The results showed that a total of 86 Dof genes were identified in wheat genome,they were unevenly distributed on the wheat chromosomes,and there were 30,29 and 27 Dof genes were distributed in A,B and D sub-genomes.Phylogenetic analysis showed that wheat Dof transcription factors could be divided into seven sub-groups,namely A,B,C1,C2,C3,D1 and D2,and D1 sub-group was the largest one with 29 members.The results of the multiple sequence alignment showed that all wheat Dof transcription factors contained a C-X₂ -C-X₂₁ -CX₂ -C motif.Collinearity analysis showed that 29 collinear gene pairs were formed by 23 Dof genes,indicating that duplication occurred during the evolution process.The expression patterns showed that Dof genes were differentially expressed in different tissues and under different abiotic stresses,indicating that Dof genes played important roles in plant growth and development,as well as in response to biotic and abiotic responses.

Key words: Wheat, Dof transcription factor, Genome-wide identification, Expression pattern

摘要： 利用生物信息学方法对小麦Dof转录因子家族进行全基因组鉴定，并对其保守结构域、系统进化关系、共线性和表达模式进行分析,以期为小麦Dof转录因子的功能研究奠定基础。结果表明，在小麦基因组水平上共鉴定到86个Dof基因，它们在小麦染色体上不均等分布，A、B、D染色体组中分别含有30、29、27个Dof转录因子；小麦Dof转录因子可被分成A、B、C1、C2、C3、D1、D2七个亚组，其中亚组D1是最大的一个亚组，含有29个Dof转录因子；多序列比对结果显示，所有的Dof转录因子都包含有一个C-X₂-C-X₂₁-CX₂-C保守基序；共线性结果表明，有23个Dof基因组成了29对共线性基因对，暗示Dof基因在进化过程中发生了复制；表达模式分析表明，Dof基因在不同生长发育时期的组织和胁迫条件下均存在差异表达，说明Dof基因广泛参与小麦生长发育，且在响应生物和非生物胁迫中可能发挥重要作用。

关键词: font-family:宋体, ">小麦；Dof转录因子；全基因组鉴定；表达模式