为了明确提高籽粒中糖含量、促进糯玉米富硒安全生产的最佳喷硒量，采用二因素裂区设计，对2个糯玉米品种彩甜糯1965（CTN1965）和晋糯20（JN20）在5个供硒水平下的生理特性及籽粒中糖、硒含量进行了比较研究。结果表明，随着叶面喷硒量增加，CTN1965和JN20的POD活性、GSH含量、叶绿素含量、可溶性糖含量、蔗糖含量均先升高后降低，以叶面喷硒45 g/hm²处理最高，分别较不喷硒对照显著提高47.94%、149.80%、49.86%、30.81%、29.33%和49.16%、115.35%、73.12%、28.08%、21.57%；MDA含量先降低后升高，以叶面喷硒45 g/hm²处理最低，较对照显著降低41.51%和40.96%；籽粒硒含量提高，以叶面喷硒90 g/hm²处理最高，分别较对照提高1 046.06%和908.76%，超过了富硒玉米的安全标准。通过方程拟合，得出CTN1965、JN20叶面喷硒量的安全上限值分别为63.96、89.53 g/hm²。综合考虑生理特性及籽粒中糖、硒含量，CTN1965、JN20叶面最佳喷硒量均为45 g/hm²，可达到富硒玉米标准。

In order to determine the optimal foliar spraying amount of selenium for improving the sugar content in grain and promoting the safe production of selenium-enriched waxy corn,two-factor split-plot design was used to study the physiological characteristics and the contents of sugar and selenium of two maize varieties Caitiannuo 1965 and Jinnuo 20 under five selenium levels.The results showed that as the spraying amount of selenium on the leaves increased,the POD activities,contents of GSH,chlorophyll,soluble sugar and sucrose of CTN1965 and JN20 all increased first and then decreased,which were the highest in the treatment with 45 g/ ha selenium,increased significantly by 47.94%,149.80%,49.86%,30.81%,29.33% and 49.16%,115.35%,73.12%,28.08%,21.57% compared with control without selenium;MDA content decreased first and then increased,which was the lowest in the treatment with 45 g/ha selenium,decreased significantly by 41.51% and 40.96% compared with control;selenium content of grain increased,which was the highest in the treatment with 90 g/ha selenium,increased by 1 046.06% and 908.76% respectively,and exceeded the safety standard of selenium-enriched corn.Through equation fitting,the safety upper limit values of spraying amount of selenium of CTN1965 and JN20 were 63.96 g/ha and 89.53 g/ha respectively.Considering the physiological characteristics and the contents of sugar and selenium in grain, the optimal spraying amount of selenium of CTN1965 and JN20 was 45 g/ha, which could reach the standard of selenium-enriched corn.