为了解河南省猪胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae,APP）的耐药情况，分离、鉴定了河南省不同地区APP菌株，采用纸片扩散法对分离菌株进行了15种药物的敏感性试验，并根据药敏试验结果对四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetH、tetG、tetK、tetL、tetM、tetO进行了PCR扩增和测序。结果显示，成功分离出5株Ⅰ型APP菌株；APP分离菌株对头孢噻肟、头孢曲松、左氧氟沙星、氧氟沙星和磺胺甲噁唑等药物敏感，对四环素（5/5，100%）、青霉素（3/5，60%）、氨苄青霉素（3/5，60%）和甲硝唑（3/5，60%）耐药；耐药基因tetB和tetA的检出率为100%（5/5）。以上结果提示，5株APP分离菌株具有多重耐药性，且其四环素耐药性主要由药物外排机制介导。

To investigate antibiotic resistence of Actinobacillus pleuropneumoniae(APP),APP isolates were isolated and identified from different regions in Henan Province.Susceptibility of 15 antibiotics for APP was determined with K-B disc method,then tetracyline-resistant genes tetA,tetB,tetC,tetD,tetE,tetH,tetG,tetK,tetL,tetM and tetO were detected and sequenced. The results showed that 5 type Ⅰ APP clinic strains were isolated.Susceptibility test showed that APP strains were sensitive to cefotaxime,ceftriaxone,levofloxacin, ofloxacin and sulfamethoxazole, and resistant to tetracycline (5/5, 100%),penicillin(3/5,60%),ampicillin(3/5,60%) and metronidazole(3/5,60%).The drug resistance genes tetB and tetA were detected(5/5,100%).The above results indicated that tetracyline-resistance of these APP strains was mediated by drug efflux.