为筛选遮阴栽培条件下适宜3年生三七生长的透光率和24-表芸苔素内酯(24-epibrassinolide,EBR)质量浓度,采用田间试验,利用遮阳网进行遮阴形成5个不同的透光率(57.31%、31.61%、18.90%、11.44%、8.04%),并设置6个质量浓度的EBR溶液[0(清水对照)、0.05、0.20、0.40、0.80、1.60 mg/L],探究不同透光率和不同质量浓度EBR溶液对3年生三七农艺性状、品质及产量的影响。结果显示,不同处理下三七形态指标不同,透光率为18.90%,三七株高、中叶长、中叶宽及单株鲜质量显著高于其他处理,分别为33.67 cm、14.06 cm、4.67 cm、53.86 g,总皂苷含量较高(8.14%);不同透光率处理对三七水分、灰分、浸出物及总皂苷含量影响不显著。EBR溶液质量浓度为0.05 mg/L时,三七株高、根冠比、水分含量和灰分含量最高,分别为32.41 cm、2.15、6.50%和5.22%;EBR质量浓度为0.20、0.40 mg/L时,三七叶柄长和叶绿素相对含量分别最高,为10.36 cm和47.21;EBR质量浓度为0.80 mg/L时,人参皂苷Rg1和单株产量最高,分别为4.00%和46.98 g,总皂苷含量较高,为7.30%。综上,透光率为18.90%更适宜3年生三七地下部分生物量与皂苷的积累;EBR质量浓度为0.80 mg/L时对三七的提质增产效果最好。
In order to screen out the light transmittance and 24-epibrassinolide (EBR) concentration suitable for triennial Panax notoginseng growth, shading network was used to set five different light transmittance(57.31%,31.61%,18.90%,11.44%,8.04%) and six concentrations of EBR solution[0(water),0.05, 0.20, 0.40, 0.80, 1.60 mg/L] was set up to explore the effect of different light transmittance and different concentrations of EBR solution on agronomic characteristics,quality and yield of triennial Panax notoginseng in field experiment.The results showed that the morphologic indexes of Panax notoginseng were different under different treatments,when the light transmittal rate was 18.90%,the plant height,middle leaf length,middle leaf width and fresh weight per plant of Panax notoginseng were significantly higher than those under other treatments,which were 33.67 cm,14.06 cm,4.67 cm,53.86 g,and total saponins content was high(8.14%).The effects of different transmittance on water content,ash content,extract and total saponins contents of Panax notoginseng were not significant.When the concentration of EBR was 0.05 mg/L,the plant height,root-shoot ratio,water content and ash content of Panax notoginseng were the highest,which were 32.41 cm,2.15,6.50% and 5.22%,respectively.When EBR concentration was 0.20 and 0.40 mg/L,petiole length and relative chlorophyll content of Panax notoginseng were the highest, which were 10.36 cm and 47.21, respectively.When the concentration of EBR was 0.80 mg/L,the yield of ginseng Rg1 and individual plant was the highest (4.00% and 46.98 g,respectively),and the total saponin content was higher(7.30%).In conclusion,when the transmittance of Panax notoginseng was 18.90%,which is more suitable for the accumulation of underground biomass and saponins. When the concentration of EBR was 0.80 mg/L,the quality and yield of Panax notoginseng were the best.