为了开发奥利亚罗非鱼有关耐盐性的分子标记，采用二代测序RNA-seq方法，挖掘奥利亚罗非鱼的微卫星标记。结果显示，拼接组装获得了71 009条Unigenes，并在所有序列中共发现了10 875个SSR位点，含有位点序列的数量为8 315条，占总序列的11.71%。奥利亚罗非鱼转录组中SSR位点类型较为丰富，共识别到5种不同核苷酸重复类型和126种不同重复基序。其中，二核苷酸串联重复单元类型的含量最多（5 779个），占总位点数的53.14%；而在二核苷酸重复类型中AC/GT基序类型出现频率最高（3 954个，占36.36%）。此外，SSR位点以6次重复次数为主（3 048个），占总位点数的27.77% ；SSR位点序列长度分布于12~254 bp，其中具有高度多态性位点3 480个（占比29.14%），中等多态性位点8 462个（占比70.86%）。

In order to develop molecular markers related to salt tolerance in Oreochromis aurea,the nextgeneration sequencing RNA-seq method was used to mine microsatellite tags in O.aurea.The results showed that a total of 71 009 unigenes were obtained by splicing and assembly,and a total of 10 875 SSR loci were found in all sequences.The number of sequences contained SSR loci was 8 315,accounting for 11.71% of the total sequences.There were many types of SSR loci in the transcriptome of O.aurea,and 5 different nucleotide repeat types and 126 different repeat motifs were recognized.Among them,the content of the dinucleotide tandem repeat unit type was the most,with 5 779,accounting for 53.14% of the total number of sites.And among dinucleotide repeat types AC/GT the motif type had the highest frequency(3 954 times,36.36%).In addition,SSR loci were dominated by 6 repetitions(3 048),accounting for 27.77% of the total number of loci;SSR loci sequence length was 12—254 bp,of which 3 480 loci were highly polymorphic(29.14%),and 8 462 loci were moderate polymorphic(70.86%).