为研究外源硼对葡萄生长及抗性相关指标的影响,以1年生红地球葡萄幼苗为试材,设置营养液硼质量浓度为0、0.2、3.1、6.2 mg/L的4个处理,处理121 d后,分析不同质量浓度硼条件下葡萄幼苗的生长量、硼、可溶性糖、淀粉、可溶性蛋白质、脯氨酸、丙二醛含量、SOD(超氧化物歧化酶)、PAX(抗坏血酸过氧化物酶)、POD(过氧化物酶)、CAT(过氧化氢酶)活性等指标。结果表明,不同处理的葡萄幼苗生长出现显著差异,硼质量浓度为3.1 mg/L时有最大新梢生长量和生物质量;葡萄叶片和根系硼含量随外源硼质量浓度增加而增加,高硼(6.2 mg/L)处理的硼含量较高,叶片硼含量为448.0 mg/kg,根系硼含量为75.3 mg/kg;无硼(0 mg/L)处理的新梢生长量、根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量、叶片SOD活性、叶片CAT活性最小,根系淀粉含量、叶片丙二醛含量和POD活性最高;随着外源硼浓度的增加,根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量以及叶片CAT活性增加,根系淀粉含量、叶片POD活性降低,叶片SOD和APX活性呈先升高后降低趋势,叶片丙二醛含量呈先降低后升高趋势;高硼(6.2mg/L)处理有较高的根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量以及较高的叶片CAT活性。外源硼质量浓度与葡萄叶片和根系硼含量呈显著正相关(r=0.950 1,r=0.989 4),与根系淀粉含量呈显著负相关(r=-0.978 2),与叶片可溶性蛋白质含量呈正相关(r=0.923 3),与叶片POD活性呈负相关(r=-0.849 6),但不显著。适量的外源硼有利于葡萄幼苗生长,无硼(0 mg/L)和高硼(6.2 mg/L)处理抑制葡萄幼苗生长,并引起抗性相关的生理生化指标的变化。
In order to study the effect of boron on the growth and resistance related indicators of grapes,we chosed one-year-old Red Globe grape seedlings as test materials,and treated them by nutrient solution containing 0,0. 2,3. 1,6. 2 mg/L boron respectively.After 121 days of treatment,we analyzed the physiological and biochemical indicators of grape seedlings under different boron concentrations.Those indicators included plant growth,contents of boron,soluble sugar,starch,protein,proline and malondialdehyde,activities of SOD,PAX,POD and CAT and so on.The results showed that there were significant differences in the growth of grape seedlings treated with different concentrations of boron.When the concentration of boron was 3. 1 mg/ L,the new shoot growth and biomass accumulation were maximum.The boron content of grape leaves and roots increased with the increase of exogenous boron concentration.For the treatment of different boron,the boron contents in leaves and roots were 448.0 mg/kg and 75.3 mg/kg,respectively. The results showed that the shoot growth,root soluble sugar content,leaf soluble protein content,leaf proline content,leaf SOD activity and leaf CAT activity were the lowest,root starch content,leaf MDA content and POD activity were the highest,with the increase of exogenous boron concentration,root soluble sugar content,leaf soluble protein content,proline content,and leaf CAT activity increased,starch content in roots and POD activity in leaves decreased,SOD and APX activities increased first and then decreased,and malondialdehyde content decreased first and then increased. High boron(6.2 mg/L) treatment had higher root soluble sugar content,leaf soluble protein content,leaf proline content and CAT activity.There were significant positive correlations between the concentration of exogenous boron and the concentration of boron in grape leaves and roots(r=0.950 1,r=0.989 4),significant negative correlation between exogenous boron concentration and starch content in root system(r=-0.978 2),positive correlation between exogenous boron concentration and soluble protein content in leaves(r=0.923 3),negative correlation between exogenous boron concentration and POD activity in leaves(r=-0. 849 6),while not significant.Appropriate amount of exogenous boron promoted the growth of grape seedlings,no boron(0 mg/L)and high boron(6.2 mg/L) treatments affected the growth and resistance related physiological and biochemical indexes of grape seedlings.