为研究蜘蛛香提取物体外抗猪繁殖与呼吸综合征病毒(PRRSV)的作用，建立Marc-145细胞模型，以利巴韦林作为阳性对照药物，采用观察细胞病变法及MTT比色法，在研究蜘蛛香提取物对Marc-145细胞最大安全质量浓度的基础上，设立蜘蛛香水提物及醇提物组、利巴韦林阳性对照组、PRRSV对照组和细胞对照组，对PRRSV进行阻断、抑制和直接灭活的测定。结果显示，蜘蛛香水提取物对PRRSV的阻断、抑制作用的最高抑制率分别为44.6%、37.9%，均极显著（P<0.01）低于利巴韦林阳性对照组，且细胞出现了较明显的病变。蜘蛛香醇提取物对PRRSV的阻断、抑制作用的最高抑制率分别为107.1%、102.6%，极显著（P<0.01）高于利巴韦林阳性对照组，细胞形态基本完好。蜘蛛香水提物和醇提物对病毒直接灭活作用的最高抑制率分别为83.5%和120.9%，均极显著（P<0.01）高于利巴韦林阳性对照组。可见，蜘蛛香醇提物抗PRRSV的作用较强。

In order to study the anti-porcine reproductive and respiratory syndrome virus (PRRSV) effect of spider fragrant extracts,a Marc-145 cell model was established,and ribavirin was used as a positive control drug.By observing cell lesions and MTT colorimetry,on the basis of the maximum safe concentration of the extracts of Valeriana jatamansi Jones on Marc-145 cells,the water extract and the alcohol extract of Valeriana jatamansi Jones groups,a ribavirin positive control group,PRRSV and cells control group were established.In the control group,PRRSV was blocked,inhibited and directly inactivated.The results showed that the highest inhibition rates of the extracts of Valeriana jatamansi Jones on blocking and inhibiting PRRSV were 44.6% and 37.9%,respectively,which was significantly(P<0.01) lower than that of the ribavirin positive control group,and the cells appeared more obvious lesions.The highest inhibitory rate of the blocking and inhibition of PRRSV by the alcohol extract of Valeriana jatamansi Jones group was 107.1% and 102.6%,respectively,which was significantly(P<0.01)higher than that of ribavirin positive control group,and the cell morphology was basically intact.The highest inhibitory rates of the water extract and the alcohol extract of Valeriana jatamansi Jones groups on the direct virus inactivation were 83.5% and 120.9%,respectively,which were significantly higher than those of the ribavirin positive control group(P<0.01).It can be seen that the spider mellow extract has a strong anti-PRRSV effect.