为缓解重金属Pb对烤烟的胁迫，降低Pb对烤烟的毒害作用，采用盆栽试验模拟土壤Pb污染（200mg/kg），设置不施肥（CK）、单施烟草专用复合肥（T1）、烟草专用复合肥+生物炭配施（T2）、烟草专用复合肥+生物炭+有机肥配施（T3）4种处理，研究了Pb胁迫下生物炭和有机肥配施对烤烟生物量积累、烟株根系活力、烟株Pb积累和土壤微生物数量的影响。结果表明，移栽后90 d，T3处理烤烟生物量比T1处理提高24.5%，且在相同施氮量情况下T3>T2>T1；烟株根系活力总体表现为T3>T2>T1>CK，其中，移栽后90 d T3、T2处理较CK分别高94.9%、55.1%；移栽后90 d T3处理土壤中有效态Pb、烟茎Pb含量和烟叶Pb含量分别较CK降低15.8%、40.6%和25.0%，另外，T3处理烟叶Pb含量与T1、T2处理相比分别下降了30.0%、20.9%；T3处理在土壤pH值和土壤微生物数量方面与CK和T1处理相比有显著改善。总体上，施用生物炭以及生物炭和有机肥配施可以降低重金属Pb胁迫对烤烟生长发育的影响，降低土壤有效态Pb含量，减少Pb在根系、茎、叶中的积累，提高土壤微生物数量。

In order to alleviate the stress of heavy metal Pb on flue-cured tobacco and reduce the toxicity of Pb,pot experiments were conducted to simulate Pb pollution in soil(200 mg/ kg).The threatments were setted as no fertilizer( CK),single application of compound fertilizer special for tobacco( T1),compound fertilizer special for tobacco and biochar combined application(T2),compound fertilizer special for tobacco,biochar and organic fertilizer(T3),and the effects of combined application of biochar and organic fertilizer on biomass accumulation of flue-cured tobacco,root activity,Pb accumulation of tobacco plant and soil microbial population under Pb stress.The results showed that the biomass of fluecured tobacco treated with T3 was 24.5% higher than that of T1 at 90 d after transplanting,and the biomass showed T3>T2>T1 at the same nitrogen application rate;the root activity of tobacco plant showed T3>T2>T1>CK in general,among which T3 and T2 were 94.9% and 55.1% higher than that of CK at 90 d after transplanting,respectively.At 90 d after transplanting,the available Pb content in soil,Pb content of tobacco stems and tobacco leaf were reduced by 15.8%,40.6% and 25.0% compared with CK,respectively,and the Pb content of tobacco leaves in T3 decreased by 30.0% and 20.9% compared with T1,T2 respectively.Soil pH value and microbial flora of T3 were significantly improved compared with CK and T1.In general,the application of biochar and the combined application of biochar and organic fertilizer could reduce the effect of heavy metal Pb on the growth of flue-cured tobacco,decrease the content of available Pb in the soil and the accumulation of Pb in roots,stems and leaves,and improve soil microorganism quantity.