玉米脱落酸受体ZmPYL9基因的克隆及功能探究

王延召; 鲁晓民; 魏良明; 周波; 黄保

doi:10.15933/j.cnki.1004-3268.2020.01.003

河南农业科学 >

2020 , Vol. 49 >Issue 1: 18 - 23

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2020.01.003

作物栽培·遗传育种

玉米脱落酸受体ZmPYL9基因的克隆及功能探究

王延召 ,
鲁晓民 ,
魏良明 ,
周波 ,
黄保

展开

河南省农业科学院粮食作物研究所，河南郑州 450002

王延召（1978-），男，河南禹州人，副研究员，博士，主要从事玉米遗传育种研究。E-mail:yz9839@126.com

收稿日期: 2019-05-15

网络出版日期: 2020-01-15

基金资助

河南省重大科技专项（151100111100）

收起

Cloning and Function Analysis of Maize Abscisic Acid Receptor ZmPYL9 Gene

Expand

(Cereal Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received date: 2019-05-15

Online published: 2020-01-15

Fold

摘要

脱落酸（ABA）参与植物发育过程以及调控多种非生物胁迫的适应性，PYL基因作为ABA受体，直接影响ABA的生物合成。为了探究玉米中PYL家族基因的功能，克隆了一个ZmPYL9基因，该基因编码197个氨基酸；物种同源蛋白质系统进化分析发现，ZmPYL9与高粱同源蛋白相似度甚高，且具有相同的保守基序；启动子顺式作用元件分析发现，ZmPYL9基因ATG上游2 000 bp区域含有多种激素应答相关的结合位点；ZmPYL9基因组织表达分析结果表明，该基因属于组成型表达，在胚乳中高度表达。ZmPYL9基因正向响应外源ABA诱导，恢复正常培养后，该基因表达量急速下降且低于CK；但是ZmPYL9基因受PEG和PEG+ABA负向诱导，且在PEG+ABA胁迫下该基因表达量高于PEG胁迫下，恢复正常培养后，2种胁迫下该基因的表达量均表现上升趋势。说明ZmPYL9基因响应干旱胁迫和ABA诱导，且ABA可以提高干旱胁迫下该基因的表达量。

关键词： 玉米; 脱落酸受体; 组成型表达; 干旱胁迫; ABA诱导

本文引用格式

王延召 , 鲁晓民 , 魏良明 , 周波 , 黄保 . 玉米脱落酸受体ZmPYL9基因的克隆及功能探究[J]. 河南农业科学, 2020 , 49(1) : 18 -23 . DOI: 10.15933/j.cnki.1004-3268.2020.01.003

Abstract

Abscisic acid(ABA)participates in plant development and regulates the adaptability of various abiotic stresses.The PYL gene acts as an ABA receptor and directly affects the biosynthesis of ABA.In order to explore the function of the PYL family gene in maize,a ZmPYL9 gene was cloned,encoding 197 amino acids.Evolutionary analysis of homologous proteins revealed that ZmPYL9 was highly homologous to sorghum homologous proteins and had the same conserved motif. Analysis of the promoter cis-acting element revealed that the 2 000 bp upstream of the ATG of the ZmPYL9 gene contained multiple hormoneresponsive binding sites.The tissue expression analysis of ZmPYL9 gene indicated that the gene was constitutively expressed and highly expressed in endosperm.The positive response of ZmPYL9 gene was induced by exogenous ABA,and the expression of this gene decreased rapidly and was lower than CK level after returning to normal rehydration culture.The ZmPYL9 gene was negatively induced by PEG and PEG+ABA,and the gene expression was higher than PEG stress under PEG+ABA stress. However,after normal rehydration,the expression of this gene under both stresses showed an upward trend.These indicate that ZmPYL9 gene responds to drought stress and ABA induction,and ABA can increase the expression of this gene under drought stress.

Key words： Maize; Abscisic acid receptor; Constitutive expression; Drought stress; ABA induction

参考文献

［1］ZHU J K.Salt and drought stress signal transduction in plants［J］.Annu Rev Plant Biol,2002,53(1):247-273.

［2］XIONG L.Regulation of abscisic acid biosynthesis［J］.Pant Physiology,2003,133(1):29-36.

［3］WILKINSON S,DAVIES W J.ABAbased chemical signalling:The coordination of responses to stress in plants［J］.Plant Cell & Environment,2010,25(2):195-210.

［4］RAZEM F A,KEREAMY A,ABRAMS S R,et al.The RNAbinding protein FCA is an abscisic acid receptor［J］.Nature,2006,439(7074):290-294.

［5］RISK J M,MACKNIGHT R C,DAY C L.FCA does not bind abscisic acid［J］.Nature,2008,456(7223):290-294.

［6］LIU X,YUE Y,LI B,et al.Comment on “A G protein coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid” ［J］.Science,2007,315(5819):1712.

［7］RISK J M,DAY C L,MACKNIGHT R C.Reevaluation of abscisic acidbinding assays shows that Gproteincoupled receptor 2 does not bind abscisic acid［J］.Plant Physiology,2009,150(1):6-11.

［8］PANDEY S,NELSON D C,ASSMANN S M.Two novel GPCRtype G proteins are abscisic acid receptors in Arabidopsis［J］.Cell,2009,136(1):136-148.

［9］PARK S Y,FUNG P,NISHIMURA N,et al.Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins［J］.Science,2009,324(5930):1068-1071.

［10］MA Y,SZOSTKIEWICE I,KORTE A,et al.Regulators of PP2C phosphatase activity function as abscisic acid sensors［J］.Science,2009,324(5930):1064-1068.

［11］YIN P,FAN H,HAO Q,et al.Structural insights into the mechanism of abscisic acid signaling by PYL proteins［J］.Nature Structural & Molecular Biology,2009,16(12):12301236.

［12］HE Y,HAO Q,LI W Q,et al.Identification and characterization of ABA receptors in Oryza sativa［J］.PLoS One,2014,9(4):e95246.

［13］CHAI Y M,JIA H F,LI C L,et al.FaPYR1 is involved in strawberry fruit ripening［J］.Journal of Experimental Botany,2011,62(14):5079-5089.

［14］WANG Y,WANG Y,KAI W,et al.Transcriptional regulation of abscisic acid signal core components during cucumber seed germination and under Cu²⁺,Zn²⁺,NaCl and simulated acid rain stresses［J］.Plant Physiology & Biochemistry,2014,76(5):67-76.

［15］李鸿杰,王盈阁,张夏伟,等.玉米脱落酸受体基因家族的生物信息学分析［J］.核农学报,2014,29(9):1657-1667.

LI H J,WANG Y G,ZHANG X W,et al.Bioinformatic analysis for abscisic acid perceptor gene family in maize［J］.Journal of Nuclear Agricultural Sciences,2014,29(9):1657-1667.

［16］RUBIO S,ANTONI R,PARK S Y,et al.In vitro reconstitution of an abscisic acid signalling pathway［J］.Nature,2009,462(7273):660-664.

［17］KIM H,HWANG H,HONG J W,et al.A rice orthologue of the ABA receptor,OsPYL/RCAR5,is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth［J］.Journal of Experimental Botany,2012,63(2):1013-1024.

［18］KIM H,LEE K,HWANG H,et al.Overexpression of PYL5 in rice enhances drought tolerance,inhibits growth,and modulates gene expression［J］.Journal of Experimental Botany,2014,65(2):453-464.

［19］XUE T,WANG D,ZHANG S,et al.Genomewide and expression analysis of protein phosphatase 2C in rice and Arabidopsis［J］.BMC Genomics,2008,9(1):550.

［20］GONZALEZ G M,PIZZIO G A,ANTONI R,et al.Arabidopsis,PYR/PYL/RCAR receptors play a major role in quantitative regulation of stomatal aperture and transcriptional response to abscisic acid［J］.The Plant Cell,2012,24(6):2483-2496.

［21］SAAVEDRA X,MODREGO A,RODRIGUEZ D,et al.The nuclear interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 is a positive regulator of abscisic acid signaling in seeds and stress［J］.Plant Physiology,2010,152(1):133-150.

［22］PIZZIO G A,RODRIGUEZ L,ANTONI R,et al.The PYL4 A194T mutant uncovers a key role of PYR1LIKE4/PROTEIN PHOSPHATASE 2CA interaction for abscisic acid signaling and plant drought resistance［J］.Plant Physiology,2013,163(1):441-455.

［23］SANTIAGO J,DUPEUX F,ROUND A,et al.The abscisic acid receptor PYR1 in complex with abscisic acid［J］.Nature,2009,462(7273):665-668.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献