猪源生物被膜乳酸菌的筛选、鉴定与体外益生特性评价

靳胜男; 舒慧萍; 张冬星; 康元环; 单晓枫; 钱爱东

doi:10.15933/j.cnki.1004-3268.2019.12.018

河南农业科学 >

2019 , Vol. 48 >Issue 12: 121 - 127

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.12.018

畜牧·兽医

猪源生物被膜乳酸菌的筛选、鉴定与体外益生特性评价

靳胜男 ,
舒慧萍 ,
张冬星 ,
康元环 ,
单晓枫 ,
钱爱东

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(吉林农业大学动物科学技术学院,吉林长春 130118）

靳胜男（1992-）,女,吉林松原人,在读硕士研究生,研究方向：动物分子免疫学。E-mail:jinsn6506@163.com

收稿日期: 2019-06-21

网络出版日期: 2019-12-15

基金资助

国家自然科学基金项目（31201927）；吉林省教育厅“十三五”科学技术项目（JJKH20180693KJ,JJKH20190910KJ）；吉林省科技厅自然科学基金学科布局项目（20170101016JC）；吉林农业大学博士启动基金项目（201801）

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Screening,Identification and Probiotic Properties Assessment of Swine Lactic Acid Bacteria with Biofilm

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(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

Received date: 2019-06-21

Online published: 2019-12-15

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摘要

将分离自健康猪新鲜粪便的乳酸菌进行功能性和安全性评价,旨在筛选出有利于猪健康生长的优良乳酸菌菌株。结果显示,共分离到2株对猪源大肠杆菌等病原菌具有抑制作用的乳酸菌菌株,并从中成功筛选出 1 株生物被膜形成能力较强的分离菌株L-8。经鉴定,分离菌株L-8为约氏乳杆菌（Lactobacillus johnsonii）,具有一定的产酸能力,能够耐受胃内pH值4.0~10.0和肠道1%胰蛋白酶环境,对猪小肠上皮细胞（IPEC-J2）具有一定的黏附性,其生存能力和生长性能均较好。此外,分离菌株L-8对小鼠无致病性,具有安全性。综上,分离菌株L-8具有作为猪用益生菌的潜在益生特性和开发前景。

关键词： 猪; 乳酸菌; 约氏乳杆菌; 生物被膜; 益生特性

本文引用格式

靳胜男 , 舒慧萍 , 张冬星 , 康元环 , 单晓枫 , 钱爱东 . 猪源生物被膜乳酸菌的筛选、鉴定与体外益生特性评价[J]. 河南农业科学, 2019 , 48(12) : 121 -127 . DOI: 10.15933/j.cnki.1004-3268.2019.12.018

Abstract

In this study,lactic acid bacteria were isolated from healthy swine stool and the function and safety of isolates were evaluated,aiming to screen lactic acid bacteria strains which were beneficial to the growth of swine.The results showed that two stains of lactic acid bacteria that exhibited antagonistic activity against some pathogens(such as Escherichia coli) were obtained. Moreover,one strain L-8 that possessed strong biofilm forming ability among the isolates was obtained.The isolated L-8 strain was identified as Lactobacillus johnsonii,and it possessed the ability of acid production,could tolerate the gastric pH value 4.0—10.0 and intestinal 1% trypsin environment.Moreover,the isolated L-8 strain had certain adhesion to porcine intestinal epithelial cells(IPEC-J2), good survival viability and growth performance.Furthermore,the isolate L-8 strain was safe and not pathogenic to mice.In conclusion,isolate L-8 strain has potential probiotic properties and development prospects as probiotics for swine.

Key words： Swine; Lactic acid bacteria; Lactobacillus johnsonii; Biofilms; Probiotic properties

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