狗枣猕猴桃AkSAP蛋白的生物信息学分析

刘丹; 李然红; 陈鑫; 王立凤

doi:10.15933/j.cnki.1004-3268.2019.12.015

河南农业科学 >

2019 , Vol. 48 >Issue 12: 103 - 108

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.12.015

园艺

狗枣猕猴桃AkSAP蛋白的生物信息学分析

刘丹 ,
李然红 ,
陈鑫 ,
王立凤

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（牡丹江师范学院，黑龙江牡丹江 157012）

刘丹（1982-），女，河南洛阳人，实验师，硕士，主要从事生物学研究。E-mail:swxld1@126.com

收稿日期: 2019-08-17

网络出版日期: 2019-12-15

基金资助

黑龙江省教育厅项目（1354MSYYB006，1354MSYYB007，1354ZD005）；牡丹江师范学院科研项目（QN2019005，YB2019005）；黑龙江药用植物开发与利用研究项目（1353PT007）；黑龙江省大学生创新创业训练计划项目（201910233007）

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Bioinformatics Analysis of AkSAP Protein from Actinidia kolomikta

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(Mudanjiang Normal University,Mudanjiang 157012,China)

Received date: 2019-08-17

Online published: 2019-12-15

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摘要

为研究狗枣猕猴桃锌指蛋白（AkSAP）的功能，利用生物信息学工具和方法，对其蛋白质的理化性质、亲水/疏水性、跨膜结构、结构功能域、二级结构、三级结构、蛋白质修饰位点及同源性等进行分析和预测。结果表明，AkSAP的cDNA全长为2 089 bp，编码区（CDS）为1 431 bp，编码蛋白为由477个氨基酸构成的非稳定亲水蛋白，属于SAP蛋白家族成员（ID：10488483），主要构件为α-螺旋和不规则卷曲，包含锌指蛋白特有的SAP结构域，与其他17种植物的SAP氨基酸序列进行对比，与中华猕猴桃变种亲缘关系较近。

关键词： 狗枣猕猴桃; 锌指蛋白; 生物信息学

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刘丹 , 李然红 , 陈鑫 , 王立凤 . 狗枣猕猴桃AkSAP蛋白的生物信息学分析[J]. 河南农业科学, 2019 , 48(12) : 103 -108 . DOI: 10.15933/j.cnki.1004-3268.2019.12.015

Abstract

To obtain information about zinc finger protein(AkSAP) from Actinidia kolomikta,the bioinformatics methods and tools were used to analyze the physical and chemical properties,hydrophobicity/hydrophilicity,transmenbranne structure,structure and function domain,secondary structure,tertiary structure,protein modification sites and homology of AkSAP from Actinidia kolomikta.The results showed that,the length of AkSAP cDNA was 2 089 bp with 1 431 bp coding area to code 477 amino acids. AkSAP was a unstable and hydrophilic protein belonging to the SAP superfamily(ID:10488483).The analysis also demonstrated that main component of AkSAP was alpha-helix and random coil and it contained the SAP domain which was specific for zinc finger protein. Compaired to SAPs of other 17 plants, AkSAP had near relationship with Actinidia chinensis var.chinensis.

Key words： Actinidia kolomikta; Zinc finger protein; Bioinformatics

参考文献

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