不同肉质颜色萝卜ANS基因表达差异分析

刘红芳; 陈发波; 李文博; 方平

doi:10.15933/j.cnki.1004-3268.2019.12.014

河南农业科学 >

2019 , Vol. 48 >Issue 12: 98 - 102

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.12.014

园艺

不同肉质颜色萝卜ANS基因表达差异分析

刘红芳 ,
陈发波 ,
李文博 ,
方平

展开

(长江师范学院现代农业与生物工程学院，重庆涪陵 408100)

刘红芳（1978-），女，甘肃武威人，讲师，硕士，主要从事萝卜病虫害防治研究。E-mail：lhongfang@163.com

收稿日期: 2019-08-10

网络出版日期: 2019-12-15

基金资助

重庆市科委基础与前沿研究项目（cstc2016jcyjA0136）;重庆市科委社会民生项目(cstc2017shms-xdny80074)

收起

Differential Expression Pattern of ANS Genes in Different Color Radish(Raphanus sativus L.)

Expand

(Modern Agriculture and Bioengineering School,Yangtze Normal University,Fuling 408100,China)

Received date: 2019-08-10

Online published: 2019-12-15

Fold

摘要

为了解不同肉质颜色萝卜ANS基因的表达量与萝卜色素含量的关系，以16份不同肉质颜色萝卜为材料，采用荧光定量PCR法检测cDNA样本中的ANS基因表达量，同时测定萝卜肉质根的色素含量，分析萝卜色素含量与ANS基因表达量的相关关系。结果表明，16份不同肉质颜色萝卜间色素含量存在极显著差异（F=114.294 0，P=0.000 1）。其中，红皮红心萝卜肉质根色素含量（23.80‰）显著高于白皮白心萝卜色素含量（0.10‰）；16份不同肉质颜色萝卜ANS基因表达量也存在极显著差异（F=95.984 0，P=0.000 1），其中，红皮红心萝卜ANS基因表达量最高（6.439 0），白皮白心萝卜ANS基因表达量最低（0.126 8）。相关分析结果表明，萝卜ANS基因表达量与萝卜色素含量的相关系数为0.83，且达到极显著水平。

关键词： 萝卜; 色素含量; ANS基因; 相关性

本文引用格式

刘红芳 , 陈发波 , 李文博 , 方平 . 不同肉质颜色萝卜ANS基因表达差异分析[J]. 河南农业科学, 2019 , 48(12) : 98 -102 . DOI: 10.15933/j.cnki.1004-3268.2019.12.014

Abstract

To explore the relationship between expression level of ANS gene and pigment content in different color radish,sixteen radish varieties with different color fleshy roots were collected as materials and their expression levels of ANS gene were detected from cDNA samples using real-time PCR technology,as well as their pigments content were determined.Subsequently,the relationship between expression level of ANS gene and the content of pigment was explored.The results showed that,the content of pigment(F=114.294 0,P=0.000 1) and expression level of ANS gene(F=95.984 0,P=0.000 1)were both significantly changed in different color radish respectively. Especially it was found that the content of pigment and expression level of ANS gene were both significantly higher in red radish(23.80‰,6.439 0)than those in white radish(0.10‰,0.126 8); In addition,the correlation analysis between expression level of ANS gene and content of pigment in different color radish was conducted,and it was found that their correlation reached extremely significant level and correlation coefficient was 0.83.it was inferred that ANS gene might be one of key regulators in pigment synthesis of radish.

Key words： Radish(Raphanus sativus L.); Pigment content; ANS gene; Correlation

参考文献

［1］陶红英,吕发生,谭革新,等.红心萝卜肉质根性状间的关系［J］.西南农业学报,2012,25(2):605-608.

［2］王丽霞,王林松,孙向东,等.心里美萝卜营养生长期肉质根过氧化物酶同工酶谱及组化分析［J］.中国生物化学与分子生物学报,2009,25(1):65-71.

［3］刘海军，孙井坤.萝卜红色素的提取工艺研究［J］.黑龙江八一农垦大学学报,2014,26(1):55-58.

［4］LIM S H,SONG J H,KIM D H,et al.Activation of anthocyanin biosynthesis by expression of the radish

r2r3myb transcription factor genersmyb1［J］.Plant Cell Reports,2016,35(3):641-653.

［5］WANG Z,WEI P,HE C,et al.Blueberry anthocyaninenriched extracts attenuate fine particulate matter(pm2.5)induced cardiovascular dysfunction［J］.Journal of Agricultural & Food Chemistry,2017,65(1):87-94.

［6］YE J B,XU F,WANG G Y,et al.Molecular cloning and characterization of an anthocyanidin synthase gene in Prunus persica(L.)［J］.Notulae Botanicae Horti Agrobotanici ClujNapoca,2017,45(1):18424309.

［7］SHI Q Q,ZHOU L,LI K,et al.Transcriptional regulation involved in anthocyanin biosynthesis in plants［J］.Forest Research,2015,35(8):42-50.
［8］赵志常,陈亚渊,高爱平,等.芒果ANS基因的克隆及其序列分析［J］.华北农学报,2014,29(S):6-9.

［9］ZHANG J,HAN Z Y,TIAN J,et al.The expression level of anthocyanidin synthase determines the anthocyanin content of crabapple(Malussp.) petals［J］.Acta Physiologiae Plantarum,2015,37(6):109.

［10］LI P,CHEN B,ZHANG G,et al.Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula

bHLH transcription factor MtTT8［J］.New Phytologist,2016,210(3):905-921.

［11]李珍,刘金兵,习卫平,等.辣椒花青素生物合成相关基因的表达分析研究［J］.华北农学报,2014,29(4):87-92.

［12］冯文亭.红早酥梨花色素苷合成相关基因克隆及功能分析［D］.杨凌:西北农林科技大学,2014.

［13］王雪,曲柏宏,鹿艳新,等.苹果梨果皮花色素苷合成相关基因PyANS的克隆与表达分析［J］.北方园艺,2016(5):108-112.
［14］孙梓健.红叶芥花青素合成途径结构基因的克隆及表达分析［D］.重庆:西南大学,2014：3-6.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献