为了鉴定控制玉米穗三叶叶宽的主效QTL，以玉米自交系郑58和D863F为亲本，构建了包含241个家系的重组自交系群体，对河南原阳、西平以及海南乐东3个环境下的玉米穗三叶叶宽进行表型测定，利用215对SSR标记构建遗传图谱，对3个环境下的玉米穗三叶叶宽进行QTL定位研究。结果表明，郑58与D863F的穗三叶叶宽存在极显著差异，且RIL群体中穗三叶叶宽表现出连续变异，基本符合正态分布，属于典型的数量性状。3个环境下的穗三叶叶宽共定位到17个QTL，单个QTL解释表型变异率为5.30%～12.77%。其中，有3个QTL分别在2个及以上环境同时检测到，是控制穗三叶叶宽的主效QTL。通过Meta-QTL分析共得到12个mQTL，检测到的5号染色体上的qThiLW2-5位于mQTL5-1区段内，qFirLW2-6位于mQTL6-1区段内，qFirLW1-8、qFirLW2-8、qSecLW2-8位于mQTL8-1区段内。

In order to identify the major quantitative trait loci(QTL) for width of three leaves near the ear in maize,a recombinant inbred lines(RIL) population including 241 family lines which derived from Zheng 58 and D863F were used to evaluated the width of three leaves near the ear in Yuanyang,Xiping of Henan Province and Ledong of Hainan Province.The QTLs associated with width of three leaves near the ear in three environments were determined by a genetic map which was constructed by 215 pairs of SSR markers.The results suggested that width of three leaves near the ear in maize showed continuous variations with a normal distribution in RIL population and belonged to typical quantitative trait.A total of 17 QTLs were detected in three environments explaining 5.30%—12.77% of the phenotypic variation.Three major QTLs for width of three leaves near the ear were detected in more than two environments. Twelve mQTLs were obtained by Meta-QTL analysis.The qThiLW2-5 and qFirLW2-6 were located in mQTL5-1 and mQTL6-1 regions respectively,qFirLW1-8,qFirLW2-8 and qSecLW2-8 were located in mQTL8-1 region.