农业资源与环境

除氨菌复配对鸡粪堆肥除臭和腐熟效果的影响

  • 刘艳薇 ,
  • 顾欣 ,
  • 惠悦然 ,
  • 念文彩 ,
  • 纪立东 ,
  • 石伟勇
展开
  • 1.宁夏大学 农学院,宁夏 银川 750021; 2.宁夏伊品生物科技股份有限公司,宁夏 银川 750100;3.宁夏农林科学院 农业资源与环境研究所,宁夏银川 750002; 4.浙江大学 环境与资源学院,浙江 杭州 310058)
刘艳薇(1993-),女,宁夏中卫人,在读硕士研究生,研究方向:农业资源利用研究。E-mail:lyww526@163.com

收稿日期: 2019-05-24

  网络出版日期: 2019-11-15

基金资助

宁夏重点研发计划重点项目(2018BBF02004,2018NCZD0083);宁夏自然科学基金项目(NZ15051);宁夏土壤与植物营养创新团队科技创新项目

 Impact of Combined Ammonia-removal Bacteria on Chicken Manure Compost Deodorization and Decomposing Effect

Expand
  •  ( 1.College of Agriculture,Ningxia University,Yinchuan 750021,China; 2.Ningxia Eppen Biotech Co.,Ltd.,Yinchuan 750100,China; 3.Ningxia Institute of Agricultural Resources and Environment,Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China; 4.College of Environment and Resources,Zhejiang University, Hangzhou 310058,China)

Received date: 2019-05-24

  Online published: 2019-11-15

摘要

为解决单一菌对禽畜堆肥除氨效果不佳的问题,以鸡粪和糠醛渣为试验材料,采用液体发酵试验和场地试验,研究除氨细菌假单胞菌(Pseudomonas sp.,A21)、芽孢杆菌(Bacillus sp.,A38)、施氏假单胞菌(Pseudomonas stutzeri,S33)、解糖假苍白杆菌(Pseudochrobactrum saccharolyticum,S61)及其复配组合的除氨效果,探索不同除氨菌对堆肥物料腐熟进程的影响。结果表明:发酵结束后,各处理堆肥物料的pH值介于7.58~7.77,含水率下降至17.23~21.34%;A21、S33复合菌处理的堆肥物料氨气累积释放量最低,有机碳、全氮、全磷、全钾含量分别为36.02%、2.33%、2.28%、0.98%,较不加菌处理分别增加了31.84%、37.06%、6.05%、15.29%,与基础物料中鸡粪相比,堆肥物料有机碳含量降低12.57%,全氮含量增加15.35%。综上所述,假单胞菌和施氏假单胞菌复配,加快堆肥物料腐熟进程,降低物料碳、氮元素损失,提高总养分含量等方面均优于其他单一菌或复合菌处理,可适用于鸡粪堆肥生产实践。

本文引用格式

刘艳薇 , 顾欣 , 惠悦然 , 念文彩 , 纪立东 , 石伟勇 . 除氨菌复配对鸡粪堆肥除臭和腐熟效果的影响[J]. 河南农业科学, 2019 , 48(11) : 75 -83 . DOI: 10.15933/j.cnki.1004-3268.2019.11.011

Abstract

 In order to solve the problem of poor ammonia removal effect of single strain on livestock com posting,taking chicken manure and furfural residue as test materials,liquid fermentation test and site test were used to study ammonia removal effect of Pseudomonas sp.(A21) and Bacillus sp.(A38),Pseudo monas stutzeri(S33),Pseudochrobactrum saccharolyticum(S61) and their combination and explore the effects of different ammonia removal bacteria on composting process of composting materials.The results showed that,when the fermentation was finished,the pH value of the composting materials was between 7.58 and 7.77,and the water content decreased to 17.23%—21.34%.A21,S33 combination treated composting materials had the lowest cumulative ammonia release amount,the contents of organic carbon,total nitrogen,total phosphorus and total potassium were 36.02%,2.33%,2.28%,0.98%,respectively,which increased by 31.84%,37.06%,6.05%,15.29% compared with no bacterium addition treatment,respectively.The organic carbon content of the composting material decreased by 12.57%,and the total nitrogen content increased by 15.35% compared to chicken manure in basic materials.In summary,Pseudomonas sp.and Pseudomonas stutzeri combination could accelerate the maturity process of compos ting materials,reduce the loss of carbon and nitrogen,and increase total nutrient content.These aspects are superior to single bacterium or other combination treatments,and could be applied to chicken manure composting production practice.

参考文献

[1]张朋月,沈玉君,刘树庆.畜禽粪便好氧发酵过程中挥发性气体排放差异研究[J].农业环境科学学报,2015,34(7):1378-1383.
2]刘志云.氨氮降解菌的分离鉴定及其抑制鸡粪氨气挥发效果的研究[D].北京:中国农业科学院,2015.
3]LE B M,DAVIES H E,GLYNN C,et al.Influence of probiotics on gut health in the weaned pig[J].Livestock Science,2010,133(13):179-181.
4]付晓政,史彬林,李倜宇.EM制剂对牛粪中氨气释放及微生物含量的影响[J].家畜生态学报,2014,35(1):71-73.
5]李婉,杨承剑,梁贤威,等.禽粪便恶臭气体产生与调控的微生物学研究进展[J].中国畜牧兽医,2013,40(8):195-198.
6]KOUKI S,SAIDI N,MHIRI F,et al.Isolation and characterization of facultative mixotrophic ammoniaoxidizing bacteria from constructed wetlands[J].Journal of Environmental Sciences(China),2011,23(10):1699-1708.
7]BRIEELEY E D R,WOOD M.Heterotrophic nitrification in an acid forest soil:Isolation and characterisation of a nitrifying bacterium[J].Soil Biology and Biochemistry,2001,33(10):1403-1409.
8]叶芬霞,朱瑞芬,叶央芳.复合微生物吸附除臭剂的制备及其除臭应用[J].农业工程学报,2008,24(8):254-257.
9]MEINEN R J,KEPHART K B,GRAVES R E.Economic feasibility and evaluation of a novel manure collection and anaerobic digestion system at a commercial swine finisher enterprise[J].Biomass and Bioenergy,2014,63:10-21.
10]纪昳,孙娟,乔丹丹,等.纳氏试剂比色法测定污水中氨氮的优化研究[J].环境科学与管理,2017,42(5):123-125.
11]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.
12]张亚宁.堆肥腐熟度快速测定指标和方法的建立[D].北京:中国农业大学,2004.
13]杨玖.磺胺类抗生素与锌对堆肥过程中酶活性及微生物群落结构多样性的影响[D].杨凌:西北农林科技大学,2014.
14]田鹤.低温缺氧环境下屠宰废弃物快速堆肥复合菌剂的制备与应用基础研究[D].重庆:西南科技大学,2018.
15]张翠绵,贾楠,胡栋,等.低温型复合发酵菌剂接种鸡粪堆肥的效应[J].环境工程学报,2016,10(10):5881-5885.
16]范建华,李尚民,吴兆林.嗜热除臭型发酵菌剂筛选及其在鸡粪堆肥发酵中的应用[J].中国家禽,2018,40(18):36-39.
17]夏丽娟,汪红,李正辉,等.苏云金芽孢杆菌在固态和液态基质中的生长差别研究[J].四川大学学报(自然科学版),2009,46(5):1483-1487.
18]GUO X,HE X,ZHANG H,et al.Characterization of dissolved organic matter extracted from fermentation effluent of swine manure slurry using spectroscopic techniques and parallel factor analysis(PARAFAC)[J].Microchemical Journal,2012,102:115-122.
19]BERNAI M P,PAREDES C,SANCHEZMONEDERO M A,et al.Maturity and stability parameters of composts prepared with a wide range of organic wastes[J].Bioresource Technology,1998,63(1):91-99.
20]李杰.不同微生物菌剂对牛粪和玉米秸秆高温腐熟的影响[D].兰州:甘肃农业大学,2013.
21]XIE K,JIA X,XU P,et al.Improved composting of poultry feces via supplementation with ammonia oxidizing archaea [J].Bioresource Technology,2012,120(3):70-77.
22]王权.添加剂对猪粪好氧堆肥过程的影响及其机制研究[D].杨凌:西北农林科技大学,2018.
23]NAKASAKI K,YAGUCHI H,SASAKI Y,et al.Effects of pH control on composting of garbage[J].Waste Management & Research,1993,11(2):117-125.
24]黄向东,韩志英,石德志,等.禽畜粪便堆肥过程中氮素的损失与控制[J].应用生态学报,2010,21(1):247-254.
25]单爱丽,王帆,严红.城市生活垃圾好氧堆肥中氨气变化及其影响[J].环境工程学报,2010,28(3):91-94.
26]邵淼,杨淑英,张增强.不同处理对高含水率奶牛粪便好氧堆肥的影响[J].农业环境科学学报,2010,29(5):982-989.
27]SHI W,NORTON J M,MILLER B E,et al.Effects of aeration and moisture during windrow composting on the nitrogen fertilizer values of dairy waste composts[J].Applied Soil Ecology,1999,11(1):17-28.
28]WONG M H.Phytotoxicity of refuse compost during the process of maturation[J].Environmental Pollution Series A Ecological and Biological,1985,37(2):159-174.
29]刘颖,肖尊东,杨恒星.EM发酵菌在畜禽粪便自然堆肥中的应用研究[J].环境科学与管理,2015,40(7):80-82.
30]RICHARD T L,HAMELERS H V M,VEEKEN A,et al.Moisture relationships in composting processes[J].Compost Science & Utilization,2002,10(4):286-302.
31]DAS K,KEENER H M.Moisture effect on compaction and permeability in composts[J].Journal of Environmental Engineering,1997,123(3):275-281. 
32]LIANG Y,LEONARD J J,FEDDES J J,et al.Influence of carbon and buffer amendment on ammonia volatilization in composting[J].Bioresource Technology,2006,97(5):748-761.
33]张志莹.不同处理牛粪CO2、NH3释放影响因素的研究[D].长春:吉林农业大学,2014.
34]BARRINGTON S,CHOINIERE D,TRIGUI M,et al.Compost convective airflow under passive aeration[J].Bioresource Technology,2003,86(3):259-266.
35]李恕艳,李吉进,张邦喜,等.菌剂对鸡粪堆肥腐殖质含量品质的影响[J].农业工程学报,2016,32(S2):268-274.
36]KADIR A A,AZHARI N W,JAMALUDIN S N.Evaluation of physical,chemical and heavy metal concentration of food waste composting[C].[S.l.]:MATEC Web of Conferences,2017. 
37]刘微,霍荣,张津,等.生物质炭对番茄秸秆和鸡粪好氧堆肥氮磷钾元素变化的影响及其机理[J].水土保持学报,2015,29(3):289-294.
 
文章导航

/