小麦几丁质酶基因家族的全基因组鉴定及禾谷镰刀菌胁迫下的表达分析

徐 武; 刘建丰; 张 戈; 徐 文; 袁祖丽

doi:10.15933/j.cnki.1004-3268.2019.11.002

河南农业科学 >

2019 , Vol. 48 >Issue 11: 7 - 17

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.11.002

作物栽培·遗传育种

小麦几丁质酶基因家族的全基因组鉴定及禾谷镰刀菌胁迫下的表达分析

徐武 ,
刘建丰 ,
张戈 ,
徐文 ,
袁祖丽

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（1.河南农业大学生命科学学院，河南郑州 450002; 2.河南省农业科学院农业经济与信息研究所，河南郑州 450002; 3.河南省农业科学院作物设计中心，河南郑州 450002）

徐武（1990-），男，河南息县人，在读硕士研究生，研究方向：小麦分子生物学。E-mail:1285517656@qq.com

收稿日期: 2019-05-10

网络出版日期: 2019-11-15

基金资助

河南省基础与前沿技术研究计划项目（162300410153）

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Genome-Wide Identification and Expression Analysis under Fusarium graminearum Stress of Chitinase Gene Family in Triticum aestivum L．

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( 1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,China; 2.Agricultural Economy＆Information Ｒesearch Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3.Crop Designing Centre,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received date: 2019-05-10

Online published: 2019-11-15

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摘要

对小麦几丁质酶基因家族成员进行鉴定,并对其基因结构、保守基序、染色体分布、系统进化及禾谷镰刀菌胁迫下的表达水平进行分析，为进一步研究几丁质酶基因在小麦应答各种生物胁迫中的作用和在抗病分子育种中的应用奠定基础。结果表明，从小麦基因组中共鉴定到159个几丁质酶基因，分布在21条染色体上；小麦几丁质酶基因家族成员分为5类，隶属于GH-18和GH-19两个亚家族。GH-18亚家族包括Ⅲ、Ⅴ类，分别含有34、48个基因；GH-19亚家族包括Ⅰ、Ⅱ、Ⅳ类，分别包含52、13、12个基因。同一亚家族的成员聚类在同一分支上，并且具有相似的外显子-内含子基因结构和保守基序。在被禾谷镰刀菌侵染后，4个小麦品系中几丁质酶基因家族一些成员的表达均显著上调。

关键词： 小麦; 几丁质酶基因家族; 全基因组鉴定; 禾谷镰刀菌; 表达模式

本文引用格式

徐武 , 刘建丰 , 张戈 , 徐文 , 袁祖丽 . 小麦几丁质酶基因家族的全基因组鉴定及禾谷镰刀菌胁迫下的表达分析[J]. 河南农业科学, 2019 , 48(11) : 7 -17 . DOI: 10.15933/j.cnki.1004-3268.2019.11.002

Abstract

The members of wheat chitinase gene family were identified,and their gene structure,conserved motif,chromosome distribution,systematic evolution and expression level under Fusarium graminearum stress were analyzed,so as to lay a foundation for further study of chitinase genes function under various biotic stres ses and molecular breeding for disease resistance in wheat. The results showed that a total of 159 chitinase genes were identified，which were distributed on 21 chromosomes in wheat genome,and divided into two sub families,GH-18 and GH-19.The GH-18 subfamily consisted of Ⅲ and Ⅴ classes,which contained 34 and 48 genes,respectively.GH-19 subfamily was composed ofⅠ,Ⅱ and Ⅳ classes,which contained 52,13 and 12 genes,respectively.Members of the same subfamily were clustered on the same branch,and had similar exon intron gene structures and conserved motifs.The expression levels of some chitinase genes were up-regulated after Fusarium graminearum inoculation in four wheat genotypes．

Key words： Wheat (Triticum aestivum L.); Chitinase gene family; Genome-wide identification; Fusari um graminearum; Expression pattern

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