农业资源与环境

菌株Enterobactersp.降解除草剂阿特拉津产物测定及降解基因克隆

  • 刘丹丹 ,
  • 孙宛玉 ,
  • 刘畅 ,
  • 马浩珂 ,
  • 王瑞娇 ,
  • 隋宇凡
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  • (沈阳化工大学 环境与安全工程学院,辽宁 沈阳 110142)
刘丹丹(1981-),女,辽宁沈阳人,讲师,博士,主要从事环境污染治理和生物修复研究。E-mail:liudandan.553@163.com

收稿日期: 2019-05-21

  网络出版日期: 2019-10-15

基金资助

国家自然科学基金项目(31400481);辽宁省大学生创新创业训练计划项目(201810149023)

 

 Determination of Herbicide Atrazine Degradation Products by Strain Enterobacter sp.and Degradation Gene Cloning

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  •  (Department of Environmental and Safety Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China)

Received date: 2019-05-21

  Online published: 2019-10-15

摘要

为解析除草剂阿特拉津的生物降解过程,采用液相色谱与质谱联用技术对菌株Enterobacter sp.降解阿特拉津的产物进行检测,通过PCR扩增技术克隆降解基因,并利用GO功能富集分析对降解基因功能进行检测。在降解产物中先后检测出羟基阿特拉津、氰尿酸和尿素组分,克隆出降解基因L465-RS09425、L465-RS10465、L465-RS12445、L465-RS09100、L465-RS12200和UreA。初步推测菌株Enterobacter sp.在降解基因的作用下,经脱氯、脱烷基、脱氨基、环裂解、脱羧和脲基甲酸水解等过程,将阿特拉津逐步矿化。

本文引用格式

刘丹丹 , 孙宛玉 , 刘畅 , 马浩珂 , 王瑞娇 , 隋宇凡 . 菌株Enterobactersp.降解除草剂阿特拉津产物测定及降解基因克隆[J]. 河南农业科学, 2019 , 48(10) : 70 -76 . DOI: 10.15933/j.cnki.1004-3268.2019.10.011

Abstract

 In order to analyze the biodegradation process of herbicide atrazine,liquid chromatography mass spectrometry was used to detect the degradation products of atrazine by functional strain Enterobacter sp.The degradation gene was cloned by the PCR amplification technology,and the degradation gene func tion was detected by using the GO function enrichment analysis.Hydroxyatrazine,cyanuric acid and urea were successively detected in the degradation products,and the degradation genes L465-RS09425,L465 RS10465,L465-RS12445,L465-RS09100,L465-RS12200 and UreA were cloned.It was speculated that strain Enterobacter sp.could mineralize atrazine gradually by dechlorination,dealkylation,deamination,ring cleavage,decarboxylation and hydrolysis of allophanate under the action of degrading genes.

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