粳稻种质资源苗期耐盐相关性状的SSR标记关联分析

谢菲菲; 田普江; 马帅国; 白天亮; 陈静茹; 张倩; 田蓉蓉; 杨治伟; 田蕾; 李培富

doi:10.15933/j.cnki.1004-3268.2019.10.003

河南农业科学 >

2019 , Vol. 48 >Issue 10: 13 - 22

DOI: https://doi.org/10.15933/j.cnki.1004-3268.2019.10.003

作物栽培·遗传育种

粳稻种质资源苗期耐盐相关性状的SSR标记关联分析

谢菲菲 ,
田普江 ,
马帅国 ,
白天亮 ,
陈静茹 ,
张倩 ,
田蓉蓉 ,
杨治伟 ,
田蕾 ,
李培富

展开

（宁夏大学农学院/宁夏优势特色作物现代分子育种重点实验室，宁夏银川 750021）

谢菲菲 (1996-)，女，宁夏银川人，在读本科生，研究方向：水稻抗逆分子育种。E-mail:977854911@qq.com

收稿日期: 2019-05-25

网络出版日期: 2019-10-15

基金资助

宁夏大学大学生科技创新与人文社会科学研究国家级项目（G201710749014）；国家自然科学基金项目（31760374，31360324）；宁夏农业育种专项（2018NYYZ0302）

收起

Association Analysis between SSＲ Markers and Salt-tolerant Traits at Seedling Stage in Japonica Ｒice Germplasm Ｒesource

Expand

(College of Agriculture，Ningxia University/Ningxia Key Laboratory of Modern Molecular Breeding for Dominant and Characteristic Crops,Yinchuan 750021,China)

Received date: 2019-05-25

Online published: 2019-10-15

Fold

摘要

选用均匀分布于水稻12对染色体的38个SSR标记对160份粳稻种质资源进行检测，结合水稻种质资源苗期耐盐性评价，发掘耐盐相关性状的分子标记关联位点，以期为耐盐水稻品种的选育提供优良的中间材料和技术保障。结果表明，各耐盐相关性状间存在一定的相关性。耐盐级别与相对苗高、相对地上部鲜质量、相对根鲜质量均呈极显著正相关，与相对茎粗呈显著正相关，与叶片伤害百分率、相对根冠比均呈极显著负相关。利用38对SSR引物，共检测到192个等位基因，其中有效等位基因109.60个，平均有效等位基因数为2.88个，Shannon’s信息指数和Nei’s遗传多样性指数平均值分别为1.139 4和0.586 9。通过关联分析，共检测到11个与水稻苗期耐盐相关性状关联的SSR位点，分布于水稻1、3、4、7、8、9、10、11、12染色体上，表型贡献率介于4.10%～18.85%，其中，表型贡献率最高的等位变异是与相对地上部鲜质量相关联的RM286-7，增加耐盐级别表型效应最大的等位变异是RM7585-1，表型贡献率为6.80%，载体种质为Bertone。

关键词： 粳稻种质资源; 苗期耐盐性; SSR标记; 遗传多样性; 关联分析

本文引用格式

谢菲菲 , 田普江 , 马帅国 , 白天亮 , 陈静茹 , 张倩 , 田蓉蓉 , 杨治伟 , 田蕾 , 李培富 . 粳稻种质资源苗期耐盐相关性状的SSR标记关联分析[J]. 河南农业科学, 2019 , 48(10) : 13 -22 . DOI: 10.15933/j.cnki.1004-3268.2019.10.003

Abstract

In order to provide excellent intermediate materials and technical support for the breeding of salt-tolerant rice varieties,thirty-eight pairs of simple sequence repeat(SSR) markers which were uni formly distributed throughout rice genome were used to detect 160 japonica rice germplasm resources,and the molecular marker sites associated with salt-tolerant traits were explored.The results showed that there was certain correlation among salt-tolerant traits.Salt tolerant score was significantly positively correlated with relative plant height,relative shoot fresh weight,relative root fresh weight and relative stem diameter.Salt tolerant score was significantly negatively correlated with leaf damage percentage and relative ratio of root to shoot.A total of 192 alleles were identified at all of 38 SSＲ loci,the effective alleles number was 109.60,and the effective alleles number per marker locus was 2.88.The average values of Shannon’s in formation index and Nei’s genetic diversity index of these SSＲ markers were 1.139 4 and 0.586 9,re spectively.Eleven sites were significantly associated with salt tolerance related traits,which were located on chromosomes 1,3,4,7,8,9,10,11 and 12,and explained the phenotypic variance from 4.10% to 18.85%.ＲM286-7 that was related with relative shoot fresh weight had the highest phenotypic contribu tion rate，and ＲM7585-1 had the highest positive phenotypic effect for salt tolerant score，whose phenotyp ic contribution rate was 6.80%,and the carrier variety was Bertone．

Key words： Japonica rice germplasm resources; Salt tolerance at seedling stage; SSＲ markers; Genetic diversity; Association analysis

参考文献

［1］张翼夫,李洪文,胡红,等.盐碱地后悬挂随动式打孔通气机的设计与试验［J］.农业工程学报,2016,32(18):42-49.

［2］WANG X,WANG J G,LIU H L,et al.Influence of natural salinealkali stress on chlorophyll content and chloroplast ultrastructure of two contrasting rice (Oryza sativa L.japonica) cultivars［J］.Australian Journal of Crop Science,2013,7(2):289-292.

［3］谭煌,杨培岭,任树梅,等.脱硫副产物配合淋洗对碱化土壤团聚体含量的影响［J］.中国农业科学,2016,49(4):705-716.

［4］王静,许兴,肖国举,等.脱硫石膏改良宁夏典型龟裂碱土效果及其安全性评价［J］.农业工程学报,2016,32(2):141-147.

［5］杨劲松.中国盐渍土研究的发展历程与展望［J］.土壤学报,2008,45(5):837-845.

［6］YU J B,WANG Z C,MEIXNER F X,et al.Biogeochemical characterizations and reclamation strategies of saline sodic soil in northeastern China［J］.CleanSoil,Air,Water,2010,38(11):1010-1016.

［7］TIAN L,TAN L B,LIU F X,et al.Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon［J］.Journal of Genetics and Genomics,2011,38(12):593-601.

［8］SHAHZAD R,KHAN A L,BILAL S,et al.Inoculation of abscisic acidproducing endophytic bacteria enhances salinity stress tolerance in Oryza sativa［J］.Environmental and Experimental Botany,2017,136:68-77.

［9］WEI LX,L B S,LI X W,et al.Priming of rice (Oryza sativa L.) seedlings with abscisic acid enhances seedling survival,plant growth,and grain yield in salinealkaline paddy fields［J］.Field Crops Research,2017,203:86-93.

［10］GIMHANI D R,GREGORIO G B,KOTTEARACHCHI N S,et al.SNPbased discovery of salinitytolerant QTLs in a biparental population of rice (Oryza sativa)［J］.Molecular Genetics and Genomics,2016,291(6):2081-2099.

［11］WANG Z F,CHENG J P,CHEN Z W,et al.Identification of QTLs with main,epistatic and QTL×environment interaction effects for salt tolerance in rice seedlings under different salinity conditions［J］.Theoretical and Applied Genetics,2012,125:807-815.

［12］GRATTAN S R,ZENG L,SHANNON M C,et al.Rice is more sensitive to salinity than previously thought［J］.California Agriculture,2002,56:189-195.

［13］GHAFFARIA A,GHARECHAHIB J,NAKHODAA B,et al.Physiology and proteome responses of two contrasting rice mutants and their wild type parent under salt stress conditions at the vegetative stage［J］.Journal of Plant Physiology,2014,171:31-44.

［14］PARDO J M.Biotechnology of water and salinity stress tolerance［J］.Current Opinion in Biotechnology,2010,21:185-196.

［15］ZHOU J W,WANG F Z,DENG P,et al.Characterization and mapping of a saltsensitive mutant in rice (Oryza sativa L.)［J］.Journal of Integrative Plant Biology,2013,55(6):504-513.

［16］LIN H X,ZHU M Z,YANO M,et al.QTLs for Na⁺ and K⁺ uptake of the shoots and roots controlling rice salt tolerance［J］.Theoretical and Applied Genetics,2004,108:253-260.

［17］KIM D M,JU H G,KWON T R,et al.Mapping QTLs for salt tolerance in an introgression line population between japonica cultivars in rice［J］.Journal of Crop Science and Biotechnology,2009,12 (3):121-128.

［18］PANDIT A,RAI V,BAL S,et al.Combining QTL mapping and transcriptome profiling of bulked RILs for identification of functional polymorphism for salt tolerance genes in rice (Oryza sativa L.)［J］.Molecular Genetics and Genomics,2010,284:121-136.

［19］LEE S Y,AHN J H,CHA Y S,et al.Mapping QTLs related to salinity tolerance of rice at the young seedling stage［J］.Plant Breeding,2007,126:43-46.

［20］ALAM R,RAHMAN M S,SERAJ Z I,et al.Investigation of seedlingstage salinity tolerance QTLs using backcross lines derived from Oryza sativa L.Pokkali［J］.Plant Breeding,2011,130(4):430-437.

［21］JAVED M A,HUYOP F Z,WAGIRAN A,et al.Identification of QTLs for morphphysiological traits related to salinity tolerance at seedling stage in indica rice［J］.Procedia Environmental Sciences,2011,8:389-395.

［22］WANG Y,ZANG J P,SUN Y,et al.Identification of genetic overlaps for salt and drought tolerance using simple sequence repeat markers on an advanced backcross population in rice［J］.Crop Science,2012,52:1583-1592.

［23］陈秀辰,杨旭,成玉富,等.数量性状关联分析及其在蔬菜中的应用［J］.中国蔬菜,2012(16):1-9.

［24］YANO K,YAMAMOTO E,AYA K,et al.Genomewide association study using wholegenome sequencing rapidly identifies new genes influencing agronomic traits in rice［J］.Nature Genetics,2016,48(8):927-934.

［25］HUANG X H,WEI X H,SANG T,et al.Genomewide association studies of 14 agronomic traits in rice landraces［J］.Nature Genetics,2010,42(11):961-967.

［26］BEGUM H,SPINDEL J E,LALUSIN A,et al.Genomewide association mapping for yield and other agronomic traits in an elite breeding population of tropical rice (Oryza sativa)［J］.PLoS One,2015,10(3):e0119873.

［27］LIW T,ZHUZ W,CHEMM,et al.A natural allele of a transcription factor in rice confers broadspectrum blast resistance［J］.Cell,2017,170(1):114-126.

［28］ZHENG H,WANG J,ZHAO H,et al.Genetic structure,linkage disequilibrium and association mapping of salt tolerance in japonica rice germplasm at the seedling stage［J］.Molecular Breeding,2015,35(7):152.

［29］KUMAR V,SINGH A,MITHRA S V A,et al.Genomewide association mapping of salinity tolerance in rice (Oryza sativa)［J］.DNA Research,2015,22(2):133-145.

［30］田蕾,王娜,张雪艳,等.盐胁迫下不同粳稻品种的形态和生理特性［J］.广东农业科学,2014,41(23):1-6.

［31］马翠苹,周先容,尚进,等.四川花萼山不同海拔巴山榧树居群的遗传多样性［J］.分子植物育种,2018,16(19):6517-6524.

［32］张立娜,曹桂兰,韩龙植.利用SSR标记揭示中国粳稻地方品种遗传多样性［J］.中国农业科学,2012,45(3):405-413.

［33］陈氏秋江,党小景,刘强明,等.水稻籽粒性状的SSR关联分析［J］.中国水稻科学,2014,28(3):243-257.

［34］张晓丽,吕荣华,唐茂艳,等.东南亚国家引进水稻种质的遗传多样性和遗传结构分析［J］.植物遗传资源学报,2018,19(1):29-38.

［35］张科,魏海锋,卓大龙,等.黑龙江省近年审定水稻品种基于SSR标记的遗传多样性分析［J］.植物遗传资源学报,2016,17(3):447-454.

［36］李宪伟,郑洪亮,赵宏伟,等.苏打盐碱胁迫下粳稻芽期耐盐碱相关性状与SSR 标记关联分析［J］.华北农学报,2018,33(2):139-148.

［37］GHOMI K,RABIEI B,SABOURI H,et al.Mapping QTLs for traits related to salinity tolerance at seedling stage of rice (Oryza sativa L.):An agrigenomics study of an Iranian rice population［J］.OMICS:A Journal of Integrative Biology,2013,17(5):242-251.

［38］SABOURI H,EZAI A M,MOUMENI A,et al.QTLs mapping of physiological traits related to salt tolerance in young rice seedlings［J］.Biologia Plantarum,2009,53(4):657-662.

［39］THOMSON M J,OCAMPO M D,EGDANE J,et al.Characterizing the saltol quantitative trait locus for salinity tolerance in rice［J］.Rice,2010,3:148-160.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献