植物保护

国内外小麦种质抗条锈病评价及相关基因分子检测

  • 陈向东 ,
  • 吴晓军 ,
  • 胡铁柱 ,
  • 李笑慧 ,
  • 李淦 ,
  • 侯开心 ,
  • 茹振钢
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  • (河南科技学院小麦中心/河南省杂交小麦重点实验室/现代生物育种河南省协同创新中心,河南 新乡 453003)
陈向东(1982-),男,河南偃师人,讲师,博士,主要从事小麦遗传育种研究。E-mail:xdchen716@126.com

收稿日期: 2019-05-28

  网络出版日期: 2019-09-15

基金资助

国家自然科学基金项目(31701502);中国博士后科学基金第56批面上项目(2014M561986);河南省科技攻关项目(192102110156);河南省高等学校重点科研计划项目(13A210287)

Evaluation of Resistance to Stripe Rust and Molecular Detection of Resistant Genes in Wheat Germplasms at Home and Abroad

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  •  ( Wheat Center,Henan Institute of Science and Technology/Henan Key Laboratory of Hybrid Wheat/Henan Modern Biological Breeding Collaborative Innovation Center,Xinxiang 453003,China)

Received date: 2019-05-28

  Online published: 2019-09-15

摘要

选用染色体1BS上与抗条锈病基因Yr10(Yellow rust 10)、Yr15、Yr24共分离或紧密连锁的5个分子标记对国内外124份小麦种质进行检测,并进行4 a的田间成株期发病病级分析,以期为合理利用小麦抗病资源、合理布局抗病品种及品种抗性改良提供参考。结果表明,在供试的124份小麦种质中,29份表现中抗及以上水平,占总数的23.39%,其中表现免疫或近免疫、高抗、中抗或中抗到高抗水平的分别有9、2、18份,分别占总数的7.26%、1.61%、14.52%;83份材料表现感病,其中表现中感、中感到高感、高感的分别有4、17、62份,分别占总数的3.23%、13.71%、50.00%;12份种质表现中感到中抗,占总材料的9.68%。Yr基因分子标记检测结果表明,携带Yr10、Yr15、Yr24基因的小麦种质分别有32、28、80份,分别占供试材料的25.81%、22.58%、64.52%。29份表现中抗及以上水平的种质中,12份含有Yr10基因,包括郑育麦9987、农大189、资麦1号、攀道拉、CL0407(早)、CL0438、09智引1号、09智引2号、CARMEN、墨S139、莫斯科39和澳阿优1号;3份含有Yr15基因,包括TAMOI、攀道拉和澳阿优1号;22份含有Yr24基因,其中,济麦22、百农64、农大189、内麦836、云麦57、09智引1号、CARMEN和法国财富麦等8份达到免疫或近免疫水平;同时含有Yr10、Yr15、Yr24基因且表现抗病的种质有攀道拉和澳阿优1号。周麦22、百农矮抗58、新麦19、鄂麦19号、HUAYUN和MRYC等6份种质未检测到上述基因(分子标记)的存在,其抗性可能受其他基因控制。

本文引用格式

陈向东 , 吴晓军 , 胡铁柱 , 李笑慧 , 李淦 , 侯开心 , 茹振钢 . 国内外小麦种质抗条锈病评价及相关基因分子检测[J]. 河南农业科学, 2019 , 48(9) : 103 -110 . DOI: 10.15933/j.cnki.1004-3268.2019.09.015

Abstract

 One hundred and twenty-four wheat germplasms were evaluated using 5 molecular markers closely linked to Yr10(Yellow rust 10) ,Yr15 and Yr24,which were resistant to stripe rust on chromosome 1BS,and disease grade was analyzed at adult stage for 4 years in field,so as to provide reference for ra tional utilization of wheat disease-resistant resources,rational distribution of disease-resistant varieties and resistance improvement of wheat varieties.The results showed that among 124 wheat germplasms,29 germ plasms displayed moderate resistance or above it,accounting for 23.39%,9 germplasms reached immune or near immune level,2 germplasms reached highly resistant level,and 18 germplasms reached moderately resistant level,accounting for 7.26% ,1.61% and 14.52% respectively; while 83 germplasms displayed susceptibility,4 germplasms reached moderately susceptible level,17 germplasms reached moderately to highly susceptible level,and 62 germplasms reached highly susceptible level,accounting for 3.23%, 13.71% and 50.00%,respectively; 12 germplasms displayed moderate susceptibility to moderate resist ance,accounting for 9.68%.The detection results of Yr genes showed that 32,28 and 80 wheat germ plasms carried Yr10,Yr15 and Yr24 genes,accounting for 25.81%,22.58% and 64.52%,respectively.Among 29 resistant germplasms,12 germplasms contained Yr10 gene,including Zhengyumai 9987,Nongda 189,Zimai No.1,Pandaola,CL0407 (Early) ,CL0438,09Zhiyin No.1,09Zhiyin No.2,CARMEN, MoS139,Moscow 39 and Aoayou No.1; only 3 germplasms contained Yr15 gene,including TAMOI,Pand aola and Aoayou No.1;22 germplasms contained Yr24 gene,among which 8 germplasms reached immune or near immune level,which were Jimai 22,Bainong 64,Nongda 189,Neimai 836,Yunmai 57,09Zhiyin No.1,CARMEN and French bermude.Yr10,Yr15 and Yr24 genes were detected in Pandaola and Aoayou No.1,while the above genes were not detected in Zhoumai 22,Bainongaikang 58,Xinmai 19,Emai No.19,HUAYUN and MRYC,so their resistance to stripe rust might be controlled by other genes.

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