为建立高效稳定的小麦遗传转化体系，以小麦郑麦9023的幼胚为外植体诱导愈伤组织，研究了培养基中不同质量浓度2，4-D、KT对愈伤组织诱导和愈伤组织继代培养的影响，进而完成整个植株再生的进程。结果表明：以小麦郑麦9023的幼胚作为外植体来诱导愈伤组织，诱导效果最好的培养基是含有 3.0mg/L 2，4-D的MS培养基，出愈率最高达92.5%；愈伤组织进行继代培养的最佳培养基上是含有1.0mg/L 2, 4-D 和 0.1mg/L KT的MS培养基；最佳的悬浮培养基是含有1.0mg/L 2, 4-D 和0.1mg/L KT的MS培养基；最佳的悬浮系继代时间为16d；悬浮系中的成熟胚在基本培养基上可以发育成正常的植株。所建立的悬浮系可以用来进行小麦基因工程遗传改良和体细胞无性系突变体的筛选。
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