偏凸山羊草fast ω-醇溶蛋白基因克隆及与WDEIA相关的IgE结合抗原表位分析

河南农业科学 ›› 2019, Vol. 48 ›› Issue (4): 9-13,81.

偏凸山羊草fast ω-醇溶蛋白基因克隆及与WDEIA相关的IgE结合抗原表位分析

霍冬敖¹，杜旭烨¹，朱斌¹，杨凡²，郭娟¹

（1.贵州师范大学，贵州贵阳 550001； 2.河南省农业科学院园艺研究所，河南郑州 450002）

收稿日期:2018-10-20 出版日期:2019-04-15 发布日期:2019-04-15
通讯作者: 郭娟(1979-），女，山西文水人，副教授，博士，主要从事作物遗传育种研究。E-mail：guojuanmoon@163.com。杨凡同为通信作者
作者简介:霍冬敖（1989-），男，河南驻马店人，副教授，博士，主要从事作物遗传育种研究。E-mail：dongaohuo@163.com
基金资助:
贵州省科技厅联合基金项目（［2017］7356，［2012］21）；河南省科技开放合作项目(172PKJHM342)

Received:2018-10-20 Published:2019-04-15 Online:2019-04-15

摘要/Abstract

摘要： 克隆偏凸山羊草中的fast ω-醇溶蛋白基因，并对其序列和IgE结合抗原表位进行分析，以期了解IgE结合抗原表位在D^v和M^v基因组中的分布，并为制备单克隆抗体进行IgE结合抗原表位的定量分析奠定基础。结果表明，从偏凸山羊草中克隆获得8个fast ω-醇溶蛋白基因（KY368672—KY368679），其中，3个fast ω-醇溶蛋白（KY368672—KY368674）由D^v基因组编码，其余5个（KY368675—KY368679）由M^v基因组编码。序列分析发现，获得的fast ω-醇溶蛋白与已发表的fast ω-醇溶蛋白具有相似的序列结构，KY368672为SRQ型ω-醇溶蛋白，KY368673和KY368674为TRQ型ω-醇溶蛋白，KY368675—KY368679均为SRL型ω-醇溶蛋白。另外，在M^v基因组编码的fast ω-醇溶蛋白中，发现了与α-醇溶蛋白相似的多聚谷氨酰胺序列。IgE结合抗原表位分析表明，M^v基因组编码的fast ω-醇溶蛋白比D^v基因组编码的fast ω-醇溶蛋含有更多的IgE结合抗原表位。

霍冬敖，杜旭烨，朱斌，杨凡，郭娟. 偏凸山羊草fast ω-醇溶蛋白基因克隆及与WDEIA相关的IgE结合抗原表位分析[J]. 河南农业科学, 2019, 48(4): 9-13,81.

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