Journal of Henan Agricultural Sciences ›› 0, Vol. ›› Issue (): 102-105.

Received:2009-09-27 Online:2010-01-15

适于AFLP 分析的盾叶薯蓣 DNA的提取方法

李勇慧,耿惠敏,押辉远

洛阳师范学院生命科学系, 河南洛阳 471022

作者简介:李勇慧( 1977 -),女 ,河南孟津人, 讲师,硕士, 主要从事生物技术研究。E-mail: huiy ongli8209@126. com
基金资助:
青年科学基金项目(30800204/C050604)

Abstract

摘要： 用传统CTAB法和改良CTAB法提取盾叶薯蓣样品的基因组DNA。结果表明:传统CTAB法与改良CTAB法提取的基因组DNA完整性都比较好,但传统CTAB法获得的DNA含有较多杂质,不能被限制性内切酶消化,改良CTAB法提取的基因组DNA杂质去除彻底,条带清晰,可以完全酶切,并能获得清晰的预扩增图谱和有丰富条带稳定的AFLP指纹,完全适合于盾叶薯蓣分子标记鉴定所需。

关键词: , 盾叶薯蓣, DNA 提取, 改良CTAB 法, AFLP

李勇慧,耿惠敏,押辉远. 适于AFLP 分析的盾叶薯蓣 DNA的提取方法[J]. 河南农业科学, 0, (): 102-105.

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适于AFLP 分析的盾叶薯蓣 DNA的提取方法

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