刘宏敏，张明^*，曹秀敏，李延龙，王利亚，杨宛玉

摘要： 研究了基于ISSR-PCR扩增的DNA指纹检测韭菜种子纯度的方法。从发芽种子的嫩芽提取基因组DNA,筛选6种ISSR引物进行ISSR-PCR扩增。聚丙烯酰胺凝胶电泳检测表明,用ISSR-64引物检测平丰8号,韭菜种纯度约92%。该技术具有准确性高、时效性好、检测成本较低等特点。

关键词: 韭菜, 种子纯度, DNA 指纹, 检测技术

刘宏敏，张明，曹秀敏，李延龙，王利亚，杨宛玉. 基于 ISSR -PCR的韭菜种子纯度的DNA指纹检测技术[J]. 河南农业科学, 0, (): 96-98.

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