Journal of Henan Agricultural Sciences ›› 0, Vol. ›› Issue (): 59-62.

Received:2009-09-19 Online:2010-01-15

纤维素酶产生菌的筛选及其相互作用研究

卢月霞¹，尹会兰¹，黄慧敏¹，杨海如²

1. 河北工程大学农学院,河北邯郸 056021; 2. 井陉县农牧局, 河北井陉 051300

作者简介: 卢月霞( 1973 -),女 ,河北邯郸人, 讲师,硕士, 主要从事微生物学方面的教学和研究工作。E-mail: luy ue xia727399@163. com

Abstract

摘要： 从森林落叶土、腐烂的秸秆和农家堆肥等含有木质纤维素降解菌的样品中分离到能较好降解纤维素的菌株4株,初步判定为3株细菌,1株放线菌。各菌株产酶(CMCase)较适宜的温度均是37℃,培养基初始pH值7.0,接种量(V/V)2%,接种培养12 h的种子液酶活较高。对各菌株单独与混合培养研究表明:菌株组合D6/D7、D1/D7、D6/D7/B7和D1/D6/D7/B7在72 h的酶活均显著高于其单一菌株,其中最优组合D6/D7在72 h的酶活可达67.12 U/mL,相当于其菌株单独培养酶活的2倍。

关键词: 纤维素降解, 混合培养, 纤维素酶, 协同作用

卢月霞，尹会兰，黄慧敏，杨海如. 纤维素酶产生菌的筛选及其相互作用研究[J]. 河南农业科学, 0, (): 59-62.

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纤维素酶产生菌的筛选及其相互作用研究

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