Genome Size，Ploidy and Phylogeny of Cyperus esculentus L.

doi:10.15933/j.cnki.1004-3268.2023.01.004

Journal of Henan Agricultural Sciences ›› 2023, Vol. 52 ›› Issue (1): 34-41.DOI: 10.15933/j.cnki.1004-3268.2023.01.004

• Crop Cultivation & Genetic Breeding • Previous Articles Next Articles

Genome Size，Ploidy and Phylogeny of Cyperus esculentus L.

WANG Huiwei¹，ZHU Shixin²，ZHANG Xinyou¹，WANG Yan¹，YANG Tiegang¹，ZHANG Xiangge¹，WANG Shufeng¹，LI Chunxin¹

（1.Industrial Crops Research Institute，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China；2.School of Life Sciences，Zhengzhou University，Zhengzhou 450001，China）

Received:2022-06-07 Published:2023-01-15 Online:2023-03-07

油莎豆基因组大小、倍性和系统发育分析

王会伟¹，朱世新²，张新友¹，王艳¹，杨铁钢¹，张向歌¹，王树峰¹，李春鑫¹

（1.河南省农业科学院经济作物研究所，河南郑州 450002；2.郑州大学生命科学院，河南郑州 450001）

通讯作者: 李春鑫（1982-），男，河南滑县人，助理研究员，博士，主要从事油莎豆种质利用和育种研究。E-mail：licx2006@163.com
作者简介:王会伟（1979-），男，河南辉县人，副研究员，博士，主要从事油莎豆种质利用和示范推广工作。E-mail：whuiweiw@163.com
基金资助:
国家重点研发计划项目（2019YFD1002600）；河南省重大科技专项（211100110100）；河南省农业科学院新兴学科发展专项（2022XK04）；河南省农业科学院自主创新项目（2022ZC13）

Abstract

Abstract: In order to clarify the genome characteristics of the main varieties and characteristic germplasm of Cyperus esculentus L. in the Huang Huai region and the phylogenetic relationship between them and their related species，the genome size and ploidy of six C. esculentus L. materials were evaluated by flow cytometry and genome survey analysis，and the phylogenetic relationship between them and their related groups was analyzed based on the sequences of the external transcribed spacer（ETS） and internal transcribed spacer（ITS） of ribosomal DNA. The results of flow cytometry analysis showed that the genomes of six C.esculentus L. materials were 0.808 6—0.858 5 Gb，with an average of 0.826 4 Gb；the results of genome survey analysis showed that the genome size，proportion of repetitive sequences，GC content and heterozygous ratio of the three tuber types of C.esculentus L. were 0.697 9 Gb，81.02%，34.7% and 0.28% for Yuyousha 2，0.778 7 Gb，84.45%，36.4% and 0.08% for Yuyousha 3.and 0.790 6 Gb，83.75%，34.9% and 0.19% for YYS‑4，respectively；genome survey analysis and pollen staining results showed that the three tuber types of C.esculentus L. were triploid. Phylogenetic analysis showed that six C. esculentus L. were clustered into C.esculentus L.branch，this branch was closely related to the branch composed of C.rotundus L. and C.glomeratus L.，they formed sister group，and formed C4 plant branch with C.iria L. ，C.glomeratus L. and C.cuspidatus H.B.K.

Key words: words：Cyperus esculentus L., Genome size, Genomic ploidy, Phylogeny

摘要： 为明确黄淮地区油莎豆主栽品种和特色种质资源的基因组特性及与其近缘类群的系统发育关系，利用流式细胞仪和基因组Survey分析对6个油莎豆材料基因组大小、倍性进行评估，并基于核糖体DNA的外转录间隔区（External transcribed spacer，ETS）和内转录间隔区（Internal transcribed spacer，ITS）序列分析其与近缘类群间的系统发育关系。流式细胞仪分析结果表明，6 个油莎豆材料基因组在0.808 6~0.858 5 Gb，均值为0.826 4 Gb；基因组Survey分析结果表明，3种粒型油莎豆材料豫油莎2号、豫油莎3号、YYS-4 的基因组大小、重复序列占比、GC 含量、杂合率分别为0.697 9 Gb、81.02%、34.7%、0.28%，0.778 7 Gb、84.45%、36.4%、0.08%，0.790 6 Gb、83.75%、34.9%、0.19%；基因组Survey分析和花粉粒染色结果显示，3种粒型油莎豆材料均为三倍体。系统发育分析结果显示，6个油莎豆材料聚为油莎豆分支，该分支与香附子和头状穗莎草构成的分支形成亲缘关系较近的姐妹群，并与其余的C4植物（碎米莎草、长尖莎草等）构成C4植物分支。

关键词: 油莎豆, 基因组大小, 基因组倍性, 系统发育

CLC Number:

S565.9

WANG Huiwei, ZHU Shixin, ZHANG Xinyou, WANG Yan, YANG Tiegang, ZHANG Xiangge, WANG Shufeng, LI Chunxin. Genome Size，Ploidy and Phylogeny of Cyperus esculentus L.[J]. Journal of Henan Agricultural Sciences, 2023, 52(1): 34-41.

王会伟, 朱世新, 张新友, 王艳, 杨铁钢, 张向歌, 王树峰, 李春鑫. 油莎豆基因组大小、倍性和系统发育分析[J]. 河南农业科学, 2023, 52(1): 34-41.

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Genome Size，Ploidy and Phylogeny of Cyperus esculentus L.

油莎豆基因组大小、倍性和系统发育分析

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