Identification and Analysis of WRKY Transcription Factors Differentially Expressed in Maize under Drought⁃Rewatering Treatment

doi:10.15933/j.cnki.1004-3268.2022.08.002

Abstract

Abstract: In order to explore WRKY transcription factors related to drought resistance in maize（Zea mays），differentially expressed WRKY genes were identified under drought⁃rewatering treatment，and their physicochemical characteristics，phylogenetic evolution，chromosome distribution，gene duplication，gene structure，conserved motif，cis⁃elements in promoter region and gene expression level were analyzed.The results showed that a total of 51 differentially expressed ZmWRKY genes were identified，the amino acid number，molecular weight and isoelectric point of 51 ZmWRKY proteins ranged from 99 to 729 aa，11.22 to 78.73 ku，and 4.58 to 12.26，respectively.ZmWRKY genes were divided into three groups Ⅰ，Ⅱ and Ⅲ，ZmWRKY genes of group Ⅱ were divided into Ⅱa，Ⅱb，Ⅱc，Ⅱd and Ⅱe.ZmWRKY genes were unequally distributed on 10 chromosomes，including 2 pairs of tandem duplication and 16 pairs of segmental duplication.The ZmWRKY genes contained 1—12 exons，most of ZmWRKY proteins（41）contained 2—4 conserved motifs，and WRKY members in the same group had similar motif compositions.Many cis⁃elements related to plant hormone and abiotic stress，such as ABRE，AuxRR⁃core，TCA⁃element，TC⁃rich repeats，TGACG⁃motif，LTR，MBS，TATC⁃box，P⁃box，CGTCA⁃motif，GC⁃motif，TGA⁃element and GARE⁃motif were identified in ZmWRKY gene promotor region.Under drought⁃rewatering treatment，51 ZmWRKY genes exhibited different expression patterns，among them，fifteen genes positively responded to drought stress，including ZmWRKY1，ZmWRKY10，ZmWRKY16，ZmWRKY28， ZmWRKY30， ZmWRKY33， ZmWRKY42， ZmWRKY65， ZmWRKY68， ZmWRKY78，ZmWRKY96，ZmWRKY99，ZmWRKY100，ZmWRKY102 and ZmWRKY111，and one gene negatively responded to drought stress，which were candidate genes for further study of drought response in
ZmWRKY family.

Key words: Zea mays, WRKY transcription factor, Phylogenetic evolution, Drought, Gene expression

摘要： 为了挖掘玉米抗旱相关WRKY转录因子，对干旱-复水处理下差异表达的玉米WRKY基因进行鉴定，并对其蛋白质理化性质、系统进化、染色体分布和复制关系、基因结构和蛋白质保守基序、启动子区顺式作用元件及干旱-复水条件下的表达量等进行分析。结果表明，共鉴定筛选出51个差异表达的玉米WRKY 基因，编码氨基酸数目介于99~729 个，分子质量介于11.22~78.73 ku，等电点介于4.58~12.26；WRKY基因分为Ⅰ、Ⅱ、Ⅲ组，其中Ⅱ又分为Ⅱa、Ⅱb、Ⅱc、Ⅱd、Ⅱe 5个亚组；WRKY基因不均等分布在10条染色体上，存在2对串联复制和16对片段复制；外显子数目为1~12个，大部分WRKY基因（41个）含有2~4 个保守基序，且同一组中的WRKY 成员具有相似的基序组成；启动子区含有ABRE、AuxRR-core、TCA-element、TC-rich repeats、TGACG-motif、LTR、MBS、TATC-box、P-box、CGTCA-motif、GC-motif、TGA-element、GARE-motif等植物激素及非生物胁迫相关的顺式作用元件。干旱-复水处理下，51个玉米WRKY基因呈现出不同表达模式，其中ZmWRKY1、ZmWRKY10、ZmWRKY16、ZmWRKY28、ZmWRKY30、ZmWRKY33、ZmWRKY42、ZmWRKY65、ZmWRKY68、ZmWRKY78、ZmWRKY96、ZmWRKY99、ZmWRKY100、ZmWRKY102 和ZmWRKY111 等15个基因正向响应干旱胁迫，ZmWRKY87 负向响应干旱胁迫，它们是今后深入研究玉米WRKY家族响应干旱的候选基因。

关键词: 玉米, WRKY转录因子, 系统进化, 干旱, 基因表达

CLC Number:

S513

FU Jiaxu, YAN Yali, XIE Xiaowen, ZHANG Xinyue, WEN Pengfei, GUAN Xiaokang, WANG Tongchao, WEI Li. Identification and Analysis of WRKY Transcription Factors Differentially Expressed in Maize under Drought⁃Rewatering Treatment[J]. Journal of Henan Agricultural Sciences, 2022, 51(8): 9-19.

付家旭, 闫亚丽, 谢小文, 张心悦, 温鹏飞, 关小康, 王同朝, 卫丽. 干旱-复水处理下差异表达玉米WRKY 基因的鉴定与分析[J]. 河南农业科学, 2022, 51(8): 9-19.

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