Effects of Nitrogen Application Rate and Selenium Application Method on Yield and Mineral Element Accumulation in Grain of Winter Wheat

doi:10.15933/j.cnki.1004-3268.2022.05.002

Abstract

Abstract: The effects of nitrogen（N）application rate（0，100，200，400 mg/kg，denoted as N0，N100，N200，N400，respectively）and selenium（Se）application method（applying 1 mg/kg Se in soil，spraying 50 mg/L Se，denoted as S and P respectively）on yield，mineral element content and accumulation amount in grain of winter wheat were studied by pot experiment，so as to provide a theoretical basis for rational fertilization to improve winter wheat quality.The results showed that the winter wheat yield increased
first and then decreased with the increase of N application rate under the two Se application methods，N200 treatment was the highest，followed by N100 treatment，and there was no significant difference between the two treatments.The average yield，panicle number and grain number per panicle of winter wheat of S treatment were significantly higher than those of P treatment.The Se contents in grain，glume and root of winter wheat of S treatment were generally significantly higher than those of P treatment，while the Se content in stem and leaf was on the contrary.Overall，the Se contents in winter wheat grain，glume，root，stem and leaf were the highest in N200 treatment，followed by N100 treatment. From the average of the two Se application methods，N200 treatment had the highest Se accumulation amounts in grain，root，stem and leaf，and N100 treatment had the highest Se accumulation amount in glume of winter wheat.The mean Se accumulation amounts in grain，glume and root of S treatment were significantly higher than those of P treatment，but the Se accumulation amount in stem and leaf was on the contrary.N application inhibited the migration of Se from glume to grain in winter wheat under two Se application methods.Compared with P treatment，S treatment promoted the Se migration from stem and leaf to glume and glume to grain.Under S treatment，in general，the contents of N，K（potassium），Ca（calcium），Mg（magnesium），Fe（ferrum），Mn（manganese）and Zn（zinc）in winter wheat grain were the highest in N100 treatment，while the contents of P（phosphorus），B（boron）and Cu（copper）were the highest in N400 treatment.Under P treatment，the Cu content in winter wheat grain was the highest in N200 treatment，the P，K，B，Fe，Mn and Zn contents were the highest in N100 treatment，and the N，Ca and Mg contents were the highest in N400 treatment. The average contents of N and K in winter wheat grain of S treatment were significantly higher than those of P treatment，and the average contents of Cu and Mo in winter wheat grain of P treatment were significantly higher than those of S treatment. Under S treatment，the accumulation amounts of N，K，Mg，B，Cu and Mo in winter wheat grain were the highest in N100 treatment，the accumulation amounts of P，Fe，Mn and Zn were the highest in N200 treatment，and the accumulation amount of Ca was the highest in N400 treatment. Under P treatment，Fe accumulation amount was the highest in N100 treatment，Ca and Cu accumulation amounts were the highest in N200 treatment，N，B and Zn accumulation amounts were the highest in N400 treatment.The average accumulation amounts of N，P，K，Ca，Mg，B，Cu，Fe，Mn and Mo in winter wheat grain of S treatment were significantly higher than those of P treatment.The correlation analysis results showed that there were significant and extremely significant positive correlations between content and accumulation amount of Se and N in winter wheat grain，and there were significant or extremely significant positive correlations between Se，N accumulation amounts and P，K，Ca，Mg，Cu，Fe and Mn accumulation amounts. In conclusion，S treatment can improve the yield of winter wheat，promote the accumulation of N，Se and other mineral elements，and improve the nutritional quality of grain. The effect is better after application of N，application of 200 mg/kg N has the best effect on the increase of yield and Se accumulation，followed by 100 mg/kg，while application of 100 mg/kg N has the best effect on accumulation of other mineral elements.

Key words: Application of selenium in soil, Spraying selenium, Nitrogen fertilizer, Winter wheat, Yield, Nutritional quality

摘要： 通过盆栽试验，研究施氮量（0、100、200、400 mg/kg，分别记作N0、N100、N200、N400）和施硒方式（土施硒1 mg/kg、喷施硒50 mg/L，分别记作S和P）对冬小麦产量和籽粒矿质元素含量、累积量的影响，为合理施肥改善冬小麦品质提供理论依据。结果表明，2种施硒方式下，冬小麦产量均随着施氮量增加先增加后降低，以N200处理最高，N100处理次之，两者差异不显著；土施硒处理冬小麦产量、穗数、穗粒数平均值均显著高于喷施硒处理。土施硒处理冬小麦籽粒、颖壳、根硒含量总体上均显著高于喷施硒处理，茎叶硒含量反之；冬小麦籽粒、颖壳、根、茎叶硒含量总体以N200处理最高，N100处理次之。从2种施硒方式平均值来看，冬小麦籽粒、茎叶、根硒累积量均以N200处理最高，颖壳硒累积量以N100处理最高；土施硒处理籽粒、颖壳、根硒累积量平均值均显著高于喷施硒处理，茎叶硒累积量反之。2种施硒方式下，施氮均抑制冬小麦硒从颖壳向籽粒的迁移；2种施硒方式比较，土施硒处理更能促进硒从茎叶向颖壳、颖壳向籽粒的迁移。土施硒条件下，冬小麦籽粒中氮、钾、钙、镁、铁、锰、锌含量总体均以N100处理最高，磷、硼、铜含量均以N400处理最高；喷施硒条件下，冬小麦籽粒中铜含量以N200处理最高，磷、钾、硼、铁、锰、锌含量均以N100处理最高，氮、钙、镁含量均以N400处理最高。土施硒处理冬小麦籽粒中氮、钾含量平均值均显著高于喷施硒处理，喷施硒处理冬小麦籽粒中铜、钼含量平均值均显著高于土施硒处理。土施硒条件下，冬小麦籽粒中氮、钾、镁、硼、铜、钼累积量均以N100处理最高，磷、铁、锰、锌累积量均以N200处理最高，钙累积量以N400处理最高；喷施硒条件下，冬小麦籽粒中铁累积量以N100处理最高，钙、铜累积量均以N200处理最高，氮、硼、锌累积量均以N400处理最高。土施硒处理冬小麦籽粒中氮、磷、钾、钙、镁、硼、铜、铁、锰、钼累积量平均值均显著高于喷施硒处理。相关性分析结果表明，冬小麦籽粒中硒含量与氮含量呈显著正相关，硒累积量与氮累积量呈极显著正相关，且硒、氮累积量与磷、钾、钙、镁、铜、铁、锰累积量均呈极显著或显著正相关。综上，土施硒较喷施硒更能提高冬小麦产量，促进氮、硒及其他矿质元素积累，改善小麦籽粒的营养品质，施氮后效果更佳，施200 mg/kg氮对提高产量及硒累积效果最好，100 mg/kg次之，施100 mg/kg氮对其他矿质元素累积效果更好。

关键词: 土施硒, 喷施硒, 氮肥, 冬小麦, 产量, 营养品质

CLC Number:

S512

MA Hongyan, NIE Zhaojun, LIU Hongen, LI Chang, QIN Shiyu, ZHAO Peng. Effects of Nitrogen Application Rate and Selenium Application Method on Yield and Mineral Element Accumulation in Grain of Winter Wheat[J]. Journal of Henan Agricultural Sciences, 2022, 51(5): 10-22.

马红艳, 聂兆君, 刘红恩, 李畅, 秦世玉, 赵鹏. 施氮量和施硒方式对冬小麦产量和籽粒矿质元素积累的影响[J]. 河南农业科学, 2022, 51(5): 10-22.

Figures/Tables 4

References

［1］LYONS G.Biofortification of cereals with foliar selenium and iodine could reduce hypothyroidism［J］.Frontiers in Plant Science，2018，9（8）：1‑8.
［2］NEWMAN R，WATERLAND N，MOON Y，et al.Selenium biofortification of agricultural crops and effects on plant nutrients and bioactive compounds important for human health and disease prevention：A review［J］.Plant Foods for Human Nutrition，2019，74（4）：449‑460.
［3］NIE Z，ZHU J，LI J，et al.Phosphorus application alters concentrations and proportions of organic Se forms in the grain of winter wheat［J］.Journal of Plant Nutrition and Soil Science，2020，183（3）：282‑291.
［4］TAN L C，NANCHARAIAH Y V，VAN HULLEBUSCH E D，et al.Selenium：Environmental significance，pollution，and biological treatment technologies ［J］.Biotechnology Advances，2016，34（5）：886‑907.
［5］TANG W，DANG F，EVANS D，et al.Understanding reduced inorganic mercury accumulation in rice following selenium application：Selenium application routes，speciation and doses［J］.Chemosphere，2017，169：369‑376.
［6］许凌凌. 植物硒的研究进展［J］.安徽农学通报，2016，22（12）：14‑16.
XU L L. Progress in the research of selenium in plants［J］.Anhui Agricultural Science Bulletin，2016，22（12）：14‑16.
［7］SHALABY T，BAYOUMI Y，ALSHAAL T，et al.Selenium fortification induces growth，antioxidant activity，yield and nutritional quality of lettuce in salt‑affected soil using foliar and soil applications［J］.Plant & Soil，2017，421：245‑258.
［8］彭涛，赵伟峰，高燕，等.不同浓度富硒营养液对小麦硒含量及产量的影响［J］.现代农业科技，2015（11）：19‑22.
PENG T，ZHAO W F，GAO Y，et al.Effect of different concentration of selenium rich liquid on wheat grain selenium content and yield［J］.Modern Agricultural Science and Technology，2015（11）：19‑22.
［9］张鹏飞.谷子硒肥肥效试验研究［D］.保定：河北农业大学，2010.
ZHANG P F.Effects of application selenium fertilizer on millet［D］.Baoding：Hebei Agricultural University，2010.
［10］张妮，李琦，张栋，等.外源硒对滴灌小麦籽粒硒含量及产量的影响［J］.麦类作物学报，2015，35（7）：995‑1001.
ZHANG N，LI Q，ZHANG D，et al.Effect of the selenium fertilizer in drip irrigation on wheat yield and selenium content［J］.Journal of Triticeae Crops，2015，35（7）：995‑1001.
［11］KRAPP A. Plant nitrogen assimilation and its regulation：A complex puzzle with missing pieces［J］.Current Opinion in Plant Biology，2015，25：115‑122.
［12］CAKMAK I，PFEIFFER W H，MCCLAFFERTY B，et al.Biofortification of durum wheat with zinc and iron［J］.Cereal Chemistry，2010，87（1）：10‑20.
［13］常旭虹，赵广才，王德梅，等.生态环境与施氮量协同对小麦籽粒微量元素含量的影响［J］.植物营养与肥料学报，2014，20（4）：885‑995.
CHANG X H，ZHAO G C，WANG D M，et al.Effects of ecological environment and nitrogen application rate on microelement contents of wheat grain ［J］.Plant Nutrition and Fertilizer Science ，2014，20（4）：885‑895.
［14］陈玉鹏.氮素对小麦硒吸收，转运及分配动态变化特征的影响［D］.杨凌：西北农林科技大学，2015.
CHEN Y P.Effects of nitrogen application on dynamic characteristics of selenium uptake translocation and distribution in wheat ［D］.Yangling：North West Agriculture and Forestry University，2015.

［15］PILON‑SMITS E A H. Selenium in plants ［M］.America：Springer International Publishing，2017.

［16］安军妹，范晓芳，侯振安，等.施硒及不同形态磷肥对小麦硒吸收和磷肥利用率的影响［J］.中国土壤与肥料，2021（2）：234‑239.
AN J M，FAN X F，HOU Z A，et al.Effects of selenium application and different forms of phosphate fertilizer on wheat selenium absorption and phosphorus use efficiency［J］.Soil and Fertilizer Sciences in China，2021（2）：234‑239.
［17］祝姣姣，聂兆君，赵鹏，等.磷硒配施对郑麦9023硒吸收及土壤硒形态转化的影响［J］.麦类作物学报，2020，40（6）：722‑729.
ZHU J J，NIE Z J，ZHAO P，et al.Effect of phosphorus combined with selenium application on selenium absorption and the transformation of chemical forms of selenium in soil of Zhengmai 9023［J］.Journal of Triticeae Crops，2020，40（6）：722‑729.
［18］GHOSH S，SAHA J，BISWAS A K.Interactive influence of arsenate and selenate on growth and nitrogen metabolism in wheat（Triticum aestivum L.）seedlings［J］.Acta Physiologiae Plantarum，2013，35（6）：1873‑1885.
［19］陈玉鹏，彭琴，梁东丽，等.施氮对小麦硒（Ⅵ）吸收，转运和分配的影响［J］.环境科学，2017，38（2）：825‑831.
CHEN Y P，PENG Q，LIANG D L，et al.Effects of nitrogen application on selenium uptake，translocation and distribution in winter wheat［J］.Environmental Science，2017，38（2）：825‑831.
［20］REIS H P G，BARCELOS J P D，FURLANI E，et al.Agronomic biofortification of upland rice with selenium and and its relation to grain quality［J］.Journal of Cereal Science，2018，79：508‑515.
［21］REIS H P G，BARCELOS J P D，SILVA V M，et al.Agronomic biofortification with selenium impacts storage proteins in grains of upland rice［J］.Journal of the Science of Food and Agriculture，2020，100（5）：1990‑1997.
［22］TEIXEIRA L S，PIMENTA T M，BRITO F，et al.Selenium uptake and grain nutritional quality are affected by nitrogen fertilization in rice（Oryza sativa L.）［J］.Plant Cell Reports，2021，40（5）：871‑880.
［23］TEIXEIRA L S，PIMENTA T M，BRITO F A L，et al.Selenium uptake and grain nutritional quality are affected by nitrogen fertilization in rice（Oryza sativa L.）［J］. Plant Cell Reports，2021，40（5）：871‑880.
［24］陈玉鹏，梁东丽，宋卫卫，等.氮素对不同生育期小麦植株累积硒的影响［J］.植物营养与肥料学报，2016，22（2）：395‑402.
CHEN Y P，LIANG D L，SONG W W，et al.Effects of nitrogen application on selenium accumulation，translocation and distribution of winter wheat at different growth periods［J］.Journal of Plant Nutrition and Fertilizer，2016，22（2）：395‑402.
［25］鲍士旦.土壤农化分析［M］.3版. 北京：中国农业出版社，2000.
BAO S D.Soil agricultural chemistry analysis［M］.3rd ed.Beijing：China Agriculture Press，2000.
［26］XU W，YU B，ZHANG Y，et al.Single‑site SBA‑15 supported zirconium catalysts. Synthesis，characterization and toward cyanosilylation reaction［J］.Applied Surface Science，2015，325：227‑234.
［27］MALEK M，KHANI M R，ALIZADEH P，et al.Composite wasteform based on SiO₂‑PbO ‑ CaO‑ZrO₂ ‑TiO₂（‑ B₂O₃‑K₂O） parent glass with zircon as the second component［J］.Ceramics International，2009，35（4）：1689‑1692.
［28］蔡天革，范宪荣，邵冉冉，等.氮硒配施对荞麦光合特性，产量和硒质量分数的影响［J］.沈阳大学学报（自然科学版），2020，32（6）：16‑23.
CAI T G，FAN X R，SHAO R R，et al.Effects of combined application of nitrogen and selenium on photosynthetic characteristics， yield and selenium content of buckwheat［J］.Journal of Shenyang University（Natural Science），2020，32（6）：16‑23.
［29］马凤霞.氮和硒对不同甘薯品种产量和营养品质的影响［D］.泰安：山东农业大学，2018.
MA F X.Effects of nitrogen and selenium on yield and nutrient quality of different varieties of sweet potato［D］.Taian：Shandong Agricultural University，2018.
［30］IRMAK S. Effects of selenium application on plant growth and some quality parameters in peanut（Arachis hypogaea）［J］.Pakistan Journal of Biological Sciences，2017，20（2）：92‑99.
［31］李贵勇，张朝钟，何清兰，等.不同生态稻作区氮肥增产效应及其利用率［J］.中国土壤与肥料，2016（2）：85‑89，113.
LI G Y，ZHANG C Z，HE Q L，et al.Effects of nitrogen fertilizer on yield increase and utilization in different ecological rice cropping areas［J］.Soil and fertilizer in China，2016（2）：85‑99，113.
［32］杨建军，王海廷，丁永峰，等.硒肥不同施用方法对水稻硒含量及产量的影响［J］.现代农业科技，2020（18）：6‑7.
YANG J J，WANG H T，DING Y F，et al.Effects of different application methods of selenium fertilizer on selenium content and yield of rice［J］.Modern Agricultural Science and Technology，2020（18）：6‑7.
［33］宋晓珂，李宗仁，王金贵.施氮对青稞硒吸收，转运和分配的影响［J］. 土壤通报，2019，50（5）：194‑200.
SONG X K，LI Z R，WANG J G. Effects of nitrogen application on selenium uptake，translocation，and distribution in naked barley［J］.Chinese Journal of Soil Science，2019，50（5）：1196‑1202.
［34］张城铭，周鑫斌.不同施硒方式对水稻硒利用效率的影响［J］.土壤学报，2019，56（1）：188‑96.
ZHANG C M，ZHOU X B.Effects of different selenium application methods on Se utilization efficiency of rice［J］.Acta Pedologica Sinica，2019，56（1）：186‑196.
［35］BOLDRIN P F，FAQUIN V，RAMOS S J，et al.Soil and foliar application of selenium in rice biofortification［J］.Journal of Food Composition and Analysis，2013，31（2）：238‑244.
［36］陈雪，沈方科，梁欢婷，等.外源施硒措施对水稻产量品质及植株硒分布的影响［J］.南方农业学报，2017，48（1）：46‑50.
CHEN X，SHEN F K，LIANG H T，et al.Effects of exogenous Se application on rice yield，quality and plant Se distribution［J］.Journal of Southern Agriculture，2017，48（1）：46‑50.
［37］袁继超，刘丛军，俄胜哲，等.施氮量和穗粒肥比例对稻米营养品质及中微量元素含量的影响［J］.植物营养与肥料学报，2006，12（2）：183‑187，200.
YUAN J C，LIU C J，E S W，et al.Effect of nitrogen application rate and fertilizer ratio on nutrition quality and trace‑elements contents of rice grain［J］.Plant Nutrition and Fertilizer Science，2006，12（2）：183‑187，200.
［38］张木.小白菜钼硒交互效应及其机制研究［D］.武汉：华中农业大学，2013.
ZHANG M.Study on interaction of molybdenum and selenium and its mechanism of Chinese cabbage［D］.Wuhan：Huazhong Agricultural University，2013.
［39］赵宁春，张其芳，程方民，等.氮、磷、锌营养对水稻籽粒植酸含量的影响及与几种矿质元素间的相关性［J］.中国水稻科学，2007，21（2）：185‑190.
ZHAO N C，ZHANG Q F，CHENG F M，et al.Effects of nitrogen，phosphorus and Zinc on phytic acid content inrice grains and their correlation with several mineral elements［J］.Chinese Rice Science，2007，21（2）：185‑190.
［40］王建国，张佳蕾，郭峰，等.钙与氮肥互作对花生干物质和氮素积累分配及产量的影响［J］.作物学报，2021，47（9）：1666‑1679.
WANG J G，ZHANG J L，GUO F，et al.Effects of interaction between calcium and nitrogen fertilizers on dry matter，nitrogen accumulation and distribution，and yield in peanut［J］.Acta Agronomica Sinica，2021，47（9）：1666‑1679.
［41］薛欣欣，魏云霞，王文斌，等.钾、镁交互作用对橡胶幼苗生长及养分吸收的影响［J］.植物营养与肥料学报，2020，26（10）：1870‑1878.
XUE X X，WEI Y X，WANG W B，et al.Interaction of potassium and magnesium on the growth and nutrient absorption of Hevea brasiliensis seedlings［J］.Journal of Plant Nutrition and Fertilizers，2020，26（10）：1870‑1878.
［42］马迅，诸旭东，宗良纲，等.不同调控措施对酸性富硒土壤硒有效性及水稻产量性状的影响［J］.土壤，2018，50（2）：284‑290.
MA X，ZHU X D，ZONG L G，et al.Effects of mixed application of calcium magnesium phosphate and aminoacid foliar fertilizer on Se availability in acid Se‑rich soil and on trait of rice yield［J］.Soils，2018，50（2）：284‑290.

[1]	FAN Yifan, ZHANG Yanyan, WANG Yimei, LI Junzhou, DU Yanxiu, SUN Hongzheng, PENG Ting, ZHAO Quanzhi, ZHANG Jing. Effects of Temperature and Light Conditions on Yield and Quality of Indica Rice under Different Sowing Dates [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 17-27.
[2]	DU Xueli, KE Luyao, LIU Zhan, DAI Mingzhu, ZHOU Yu, LI Jianxing, XU Jiuliang. Effects of Foliar Magnesium on Yield，Quality and Contents of Anthocyanin and Metabolites of Purple Rice [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 28-36.
[3]	XU Chunyan, ZHANG Qian, WANG Yaxian, WANG Hongyu, SHENG Kai, WANG Yilun. Effects of Commercial Organic Fertilizers on Physical and Chemical Properties of Sandy Fluvo⁃Aquic Soil，Yield and Nutrient#br# Accumulation of Winter Wheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 56-64.
[4]	YANG Mingda, ZHANG Suyu, LI Shuai, ZHENG Dongfang, YANG Shenjiao, GUAN Xiaokang, WANG Tongchao. Effects of Different Sowing and Irrigation Methods on Seedling Emergence，Dry Matter Accumulation，Transport and Yield of Summer Maize [J]. Journal of Henan Agricultural Sciences, 2024, 53(1): 22-31.
[5]	MA Zhonglian, GONG jianfu, MA Yongcui, TIAN Hong, LIU Defu, YANG Shunqiang. Effects of Exogenous Melatonin on Physiological Characters，Yield and Quality of Tartary Buckwheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(1): 41-47.
[6]	KONG Fandan, ZHOU Lijun, ZHENG Meiyu, ZHANG Zuohe, YANG Zeyi, WU Juan. Effects of Straw Mulching on Soil Microbial Biomass，Enzyme Activity，and Soybean Yield in Black Soil Areas [J]. Journal of Henan Agricultural Sciences, 2024, 53(1): 87-95.
[7]	LU Xiaomin, GUO Shulei, ZHANG Xin, WEI Liangming, ZHANG Qianjin, CAO Liru, LIU Haijing, DENG Yazhou, ZHANG Zhen, WANG Zhenhua. Analysis of Yield Composition of Stem Lodging Resistance Related Traits of Maize under Different Densities [J]. Journal of Henan Agricultural Sciences, 2023, 52(9): 44-55.
[8]	LIAN Yanhao, DU Feibo, XUE Bo, WANG Zhiqiang, REN Yongzhe, LIN Tongbao, LI Sen. Effect of Bed Planting Combined with One‑time Nitrogen Fertilizer Application on Nitrogen Utilization and Economic Benefit of Winter Wheat（Triticum aestivum L.） [J]. Journal of Henan Agricultural Sciences, 2023, 52(9): 56-65.
[9]	XU Lina, AN Zhiliang, CHEN Shilin, ZHANG Huaisheng, JIN Xining, WANG Pingxi. Effect of Planting Density on Stem Strength and Ear Characters of Maize [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 11-17.
[10]	ZHAO Lishang, WANG Xiangsheng, GAO Nanan, HAN Pengbin, LI Jie, REN Yongzhe, LIN Tongbao, WANG Zhiqiang, LIAN Yanhao. Effects of Intercropping of Two Genotypes of Wheat on Grain Filling Characteristics and Yield [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 18-25.
[11]	XIE Wenxiao, JIANG Xiuying, LÜ Jun, PAN Zhengyan, HAN Yong, WANG Qingxin, LI Jianguo. Effect of Functional Leaves at Different Positions on Grain Filling Characteristics and Quality of Different Panicle Types of Rice Varieties [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 36-44.
[12]	ZHANG Licheng, LI Juan, ZHANG Mingqing, GU Zuchao. Cabbage Yield and Nutrient Utilization under Substitution of Organic Manure for Fertilizer in Vegetable‑Rice Rotation in Lateritic Red Soil [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 87-95.
[13]	DOU Dandan, SUN Jianjun, WANG Dexin, GUO Yuxi, GUO Xinhai, DING Chaoming. Effect of Sowing Date on Yield and Quality of Early and Middle Rice [J]. Journal of Henan Agricultural Sciences, 2023, 52(7): 12-23.
[14]	FAN Xiaoqing, ZHAO Xinyue, WANG Yuwen, ZHANG Liangyao, YUAN Jincheng, LIU Yinghui, WENG Qiaoyun. Effect of Intercropping of Silage Corn and Forage Rape on Forage Crop Yield and Quality [J]. Journal of Henan Agricultural Sciences, 2023, 52(7): 40-51.
[15]	ZHANG Jing, SU Sheng, MAO Wei, ZENG Hongyu, QIU Jianrong, ZHAO Haitao. Fertilization Effect of Rapeseed under Different Soil Fertilities in Region along Yangtze River in Jiangsu Province [J]. Journal of Henan Agricultural Sciences, 2023, 52(7): 60-69.