Abstract: Tobacco secretion of long-stalk secretory glandular trichomes has an important influence on the quality of tobacco leaves.In order to clarify the genetic characteristics of long-stalk glandular trichomes,we used the homozygous long-stalk secretory glandular trichome type T.I.1068 as the male parent and non-secretory glandular trichomes type T.I.1112 as the female parent to construct a population of 6 generations(P₁,P₂,F₁,F₂,BC₁and BC₂).The joint segregation analysis method of mixed major gene plus polygene genetic model was used to study the genetic characteristics of secretory and non-secretory types of long-stalk glandular trichomes.The results showed that the inheritance of the relative proportion of tobacco long-stalk secretory glandular trichomes to non-secretory glandular trichomes was controlled by two pairs of additive-dominant-epistatic major genes plus additive-dominant-epistatic-polygenic models.The heritability of the main genes of the BC₁,BC₂ and F₂ generations were 80.05%,97.88% and 96.50%,respectively.The heritability of the main genes was very high,and the selection of glandular trichomes types could be finished in the early generation; the multi-gene heritability of the BC₁,BC₂ and F₂ generation were 16.15%,1.55% and 2.75%,respectively.The impact of polygenes should be considered in the BC₁generation,while the impact of the polygenes in the BC₂and F₂ generations was negligible.Our results showed that the relative proportion of long-stalk secretory and non-secretory glandular trichomes is controlled by two pairs of main genes,mainly by major gene effects,while affected by the interaction between genes.

Key words: Tobacco, Long-stalk secretory glandular trichome, Non-glandular trichome, Ratio, Genetic analysis

摘要： 烟草长柄分泌型腺毛的分泌物对烟叶的品质有重要影响，为了明确长柄腺毛的遗传特性，以纯合长柄分泌型腺毛烟草材料T.I.1068为父本、非分泌型腺毛烟草材料T.I.1112为母本，构建6个世代的群体（P₁、P₂、F₁、F₂、BC₁、BC₂），利用主基因+多基因混合遗传模型分离分析方法，研究烟草长柄分泌型和非分泌型腺毛的遗传规律。结果表明，烟草长柄分泌型腺毛与非分泌型腺毛相对比例的遗传由2对加性-显性-上位性主基因+加性-显性上位性多基因模型控制。BC₁、BC₂和F₂世代主基因的遗传率分别为80.05%、97.88%和96.50%，主基因遗传率很高，可以在早代进行腺毛种类的选择；BC₁、BC₂和F₂世代多基因的遗传率分别为16.15%、1.55%和2.75%，BC₁代应考虑多基因的影响，BC₂和F₂代多基因的影响较小。可见，长柄分泌型与非分泌型腺毛相对比例的遗传受2对主基因控制，以主基因效应为主，受基因间的互作的影响。

关键词: 烟草, 长柄分泌型腺毛, 非分泌型腺毛, 比例, 遗传分析