Abstract: In this study,Lactobacillius was isolated from the feces of healthy adult Chinese pastoral dogs and their prebiotic characteristics were studied.Three strains of Lactobacillus were isolated from the faeces of pastoral dogs using MRS improved medium. The results of morphological observation,physiological and biochemical tests and 16S rRNA gene sequence homology analysis showed that they were identified as L.salivarius,L.johnsonii and L.helveticus, named TCSLl, TCSL2 and TCSL3 respectively.Then acid resistance test,bile salt resistance test,cell adhesion test,bacteriostatic test and oral growth promoting test were carried out in vitro.The results showed that the three strains were tolerant to 0.6% Bile salt,pH value 2.0.The adhesion rate of dog intestinal epithelial cells was more than 65%,and the supernatant had inhibitory effect on dog pathogenic E.coli.There was no diarrhea and death in mice fed for 28 days,TCSL1 and TCSL2 could significantly increase the body weight of mice (P<0.05).Three strains of Lactobacillus isolated from the feces of scattered pastoral dogs have probiotic characteristics,which can be used as candidate strains for developing probiotic preparations for dogs.

Key words: Pastoral dogs, Lactobacillus, Isolation and identification, Prebiotic characteristics, Probiotic preparation

摘要： 从散养的健康成年中华田园犬粪便中分离乳酸杆菌，研究其益生特性，旨在开发犬用益生菌菌株。采用MRS改良培养基从田园犬粪便中分离出3株乳酸杆菌，进行细菌染色形态观察、生理生化试验和16S rRNA 基因序列同源性分析，鉴定为唾液乳酸杆菌（L.salivarius）、约氏乳酸乳杆菌（L.johnsonii）和瑞士乳酸杆菌（L.helveticus），分别命名为TCSL1、TCSL2和TCSL3。随后进行体外耐酸试验、抗胆盐试验、细胞黏附试验、抑菌试验和小鼠口服促生长等益生特性试验。结果显示，3株分离菌株能耐受0.6%的胆盐、pH值2.0 的酸度；对犬肠黏膜上皮细胞黏附率均在65%以上；其上清液对犬致病性大肠杆菌均有抑制作用；连续28 d 饲喂小鼠，未见腹泻和死亡现象，TCSL1和TCSL2能显著增加小鼠体质量（P<0.05）。从散养田园犬粪便中分离到的3株乳酸杆菌具有益生特性，可作为犬用益生菌制剂的候选菌株进行开发。

关键词: 田园犬, 乳酸杆菌, 分离鉴定, 益生特性, 益生菌制剂