Abstract: In order to understand the function of wheat ABA receptor gene TaPYL9(Pyrabactin resistance like 9)under abiotic stress,TaPYL9 was isolated by homologous cloning method,the complete sequence was analyzed by bioinformatics method,and the expression levels of TaPYL9 were detected by real-time PCR under ABA,NaCl and PEG conditions.The results showed that full-length of TaPYL9 was 1 173 bp,and contained a complete open reading frame of 618 bp,encoding an unstable hydrophilic protein which was consisted of 205 amino acid residues.TaPYL9 protein was predominated by α-helix,and contained a
PYL helix structure which was composed of 2 α-helixes and 7 β-folds;TaPYL9 protein shared the highest protein sequence identity with AsPYL9-like protein in Aegilops tauschii,and it belonged to the same subfamily with AtPYL9 protein in Arabidopsis;TaPYL9 protein had the same motif with AsPYL9-like protein,and the motif similarity of TaPYL9 protein was the highest to AtPYL9 protein of all PYL proteins in Arabidopsis.Real-time PCR showed that the expression levels of TaPYL9 gene generally increased under the ABA,NaCl and PEG conditions. Overall,TaPYL9 protein is a wheat ABA receptor,which is sensitive to ABA, and maybe regulate salt and drought stress in wheat.

Key words: Wheat, ABA receptor, PYR/ PYL/RCAR, TaPYL9, Drought stress, Salt stress, ABA pathway

摘要： 为了了解小麦ABA受体基因TaPYL9（Pyrabactin resistance like 9）在非生物胁迫下的功能，通过同源克隆法获得小麦TaPYL9基因，对其进行生物信息学分析，并采用实时荧光定量PCR（qRT-PCR）技术检测该基因在ABA、NaCl和PEG处理下的表达情况。结果表明，TaPYL9 cDNA全长1 173 bp，开放阅读框618 bp，编码1个包含205个氨基酸残基的不稳定的亲水蛋白，该蛋白以α-螺旋为主，含有1个由2个α-螺旋和7个β-折叠组成的PYL螺旋手柄结构；TaPYL9蛋白与山羊草AsPYL9-like蛋白亲缘关系最近，与拟南芥AtPYL9蛋白属于同一亚族；TaPYL9蛋白和AsPYL9-like蛋白保守基序相同，与拟南芥13个PYL中的AtPYL9蛋白的保守基序相似性最高；TaPYL9在ABA、NaCl和PEG处理下总体上表达量上升。综上，TaPYL9为小麦ABA受体蛋白，对ABA敏感，可能参与小麦高盐和干旱胁迫应答的调控。

关键词: 小麦, ABA受体, PYR/PYL/RCAR, TaPYL9, 干旱胁迫, 盐胁迫, ABA通路